Molecular cloning and sequencing of partial cDNA of tumor necrosis factor and p75 tumor necrosis factor receptor of Syrian golden hamster (Mesocricetus auratus) with the use of universal primers

被引：0

作者：

Dobrzanska, Z ^{[1
]}

Wieckiewicz, J ^{[1
]}

Bigda, J ^{[1
]}

机构：

[1] Med Univ Gdansk, Dept Histol & Immunol, Intercollegiate Fac Biotechnol UGMUG, PL-80211 Gdansk, Poland

来源：

ACTA BIOCHIMICA POLONICA | 2002年 / 49卷 / 02期

关键词：

hamster; TNF; p75; TNF-R; PCR primers;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study we cloned and analysed partial cDNA of tumor necrosis factor (TNF) and p75 TNF-R receptor of Syrian golden hamster (Mesocricetus auratus). We obtained a 382-bp sequence of TNF and a 148-bp sequence coding for p75 TNF-R. The primers used for the cloning were designed on the basis of inter-species homology, thus presumably can be used for cloning and analysis of TNF and p75 TNF-R genes of other mammals.

引用

页码：427 / 431

页数：5