Molecular cloning and sequencing of partial cDNA of tumor necrosis factor and p75 tumor necrosis factor receptor of Syrian golden hamster (Mesocricetus auratus) with the use of universal primers

被引：0
|
作者：
Dobrzanska, Z [1 ]
Wieckiewicz, J [1 ]
Bigda, J [1 ]
机构：
[1] Med Univ Gdansk, Dept Histol & Immunol, Intercollegiate Fac Biotechnol UGMUG, PL-80211 Gdansk, Poland
来源：
ACTA BIOCHIMICA POLONICA | 2002年 / 49卷 / 02期
关键词：
hamster; TNF; p75; TNF-R; PCR primers;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study we cloned and analysed partial cDNA of tumor necrosis factor (TNF) and p75 TNF-R receptor of Syrian golden hamster (Mesocricetus auratus). We obtained a 382-bp sequence of TNF and a 148-bp sequence coding for p75 TNF-R. The primers used for the cloning were designed on the basis of inter-species homology, thus presumably can be used for cloning and analysis of TNF and p75 TNF-R genes of other mammals.
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收藏
页码：427 / 431
页数：5
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