Gene cloning, expression, and characterization of a novel trehalose synthase from Arthrobacter aurescens

被引：47
作者：
Wu Xiuli [1 ]
Ding Hongbiao [1 ]
Yue Ming [1 ]
Qiao Yu [1 ]
机构：
[1] Chinese Acad Agr Sci, Feed Res Inst, Beijing 100081, Peoples R China
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2009年 / 83卷 / 03期
基金：
国家高技术研究发展计划(863计划);
关键词：
Trehalose synthase; Trehalose; Arthrobacter aurescens; Escherichia coli; PURIFICATION; MALTOSE; ENZYME; BASIDIOMYCETE;
D O I：
10.1007/s00253-009-1863-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Trehalose synthase (TreS) is an intramolecular transglycosylase. It specially catalyzes the conversion of maltose and trehalose. In this study, a novel treS gene, which had a length of 1,797 bp and encoded 598 amino acids, was cloned from Arthrobacter aurescens CGMCC 1.1892 and expressed in Escherichia coli. Thin layer chromatography results indicated that it could catalyze the conversion between maltose and trehalose in one step. However, the ion chromatography results showed that, as a byproduct, about 13% glucose was also produced. The purified recombinant enzyme had a molecular weight of 68 kDa and showed its optimal activity at 35 A degrees C and pH 6.5. This enzyme was not thermostable, and its activity was increased by 1 mM Mg2+, Mn2+, and Ca2+ while strongly inhibited by 5 mM Cu2+ and SDS.
引用
收藏
页码：477 / 482
页数：6
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