Bacteriophage Cocktail for the Prevention of Multiple-Antibiotic-Resistant and Mono-Phage-Resistant Vibrio coralliilyticus Infection in Pacific Oyster (Crassostrea gigas) Larvae

被引：25

作者：

Kim, Hyoun Joong ^{[1
,2
]}

Jun, Jin Woo ^{[3
]}

Giri, Sib Sankar ^{[1
,2
]}

Kim, Sang Guen ^{[1
,2
]}

Kim, Sang Wha ^{[1
,2
]}

Kwon, Jun ^{[1
,2
]}

Lee, Sung Bin ^{[1
,2
]}

Chi, Cheng ^{[4
]}

Park, Se Chang ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Lab Aquat Biomed, Coll Vet Med, Seoul 08826, South Korea

[2] Seoul Natl Univ, Res Inst Vet Sci, Seoul 08826, South Korea

[3] Korea Natl Coll Agr & Fisheries, Dept Aquaculture, Jeonju 54874, South Korea

[4] Nanjing Agr Univ, Coll Anim Sci & Technol, Lab Aquat Nutr & Ecol, Nanjing 210095, Peoples R China

来源：

PATHOGENS | 2020年 / 9卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

pacific oyster larvae; mass mortality; Vibrio coralliilyticus; bacteriophage; phage-resistant variant; cocktail phage; PSEUDOMONAS-PLECOGLOSSICIDA; BACILLARY NECROSIS; PATHOGEN; MORTALITY; TUBIASHII; THERAPY; DISEASE; SEA;

D O I：

10.3390/pathogens9100831

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Vibrio coralliilyticus (V. coralliilyticus) is a pathogen that causes mass mortality in marine bivalve hatcheries worldwide. In this study, we used a bacteriophage (phage) cocktail to prevent multiple-antibiotic-resistant (MAR) and phage-resistant (PR) V. coralliilyticus infection in Pacific oyster (Crassostrea gigas) larvae. To prevent the occurrence of phage-resistant strains and decrease the effect of mono-phage treatment, we prepared a phage cocktail containing three types of V. coralliilyticus-specific phages and tested its prophylactic efficacy against MAR and PR V. coralliilyticus infection. The results of the cell lysis test showed that the phage cocktail showed an excellent bactericidal effect against the MAR and PR variants in contrast to the experimental group treated with two mono phages (pVco-5 and pVco-7). An in vivo test using Pacific oyster larvae also confirmed the preventive effect against MAR and PR variants. In conclusion, the application of the phage cocktail effectively prevented V. coralliilyticus infection in marine bivalve seedling production. Furthermore, it is expected to reduce damage to the aquaculture industry caused by the occurrence of MAR and PR V. coralliilyticus. Therefore, phage cocktails may be used for the control of various bacterial diseases.

引用

页码：1 / 10

页数：9

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