NEW MICROSATELLITE MARKERS FOR TRICYRTIS MACRANTHA (CONVALLARIACEAE) AND CROSS-AMPLIFICATION IN CLOSELY RELATED SPECIES

被引：2

作者：

Ohki, Noriko ^{[1
]}

Setoguchi, Hiroaki ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Human & Environm Studies, Sakyo Ku, Kyoto 6068501, Japan

来源：

APPLICATIONS IN PLANT SCIENCES | 2013年 / 1卷 / 05期

关键词：

Convallariaceae; cross-amplifi cation; genetic diversity; microsatellite; population structure; Tricyrtis macrantha;

D O I：

10.3732/apps.1200247

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Premise of the study: Microsatellite markers were characterized in Tricyrtis macrantha (sect. Brachycyrtis, Convallariaceae), a vulnerable species endemic to Japan, to investigate its genetic diversity and population structure. Methods and Results: Eleven microsatellite markers were developed. The number of alleles per locus ranged from one to seven, and genetic diversity per locus ranged from 0.142 to 0.812. Four markers were successfully amplified in other species of sect. Brachycyrtis (T. ishiiana, T. ishiiana var. surugensis, and T. macranthopsis). Conclusions: The microsatellite markers can be used to investigate the genetic diversity and population structure of the vulnerable and endangered species of Tricyrtis sect. Brachycyrtis, to aid in the development of conservation strategies for each species.

引用

页数：3

共 10 条

[1] [Anonymous], 1987, Science, Philosophy, and Human Behavior in the Soviet Union
[2] Doyle JJ., 1987, FOCUS, V19, P11, DOI DOI 10.2307/2419362
[3] FSTAT (Version 1.2): A computer program to calculate F-statistics
Goudet, J
[J]. JOURNAL OF HEREDITY, 1995, 86 (06) : 485 - 486
[4] *JAP SOC PLANT TAX, 1993, RED DAT BOOK JAP END
[5] Development of microsatellite markers in black locust (Robinia pseudoacacia) using a dual-supression-PCR technique
Lian, C
Hogetsu, T
[J]. MOLECULAR ECOLOGY NOTES, 2002, 2 (03): : 211 - 213
[6] An improved technique for isolating codominant compound microsatellite markers
Lian, Chunlan
Wadud, Md. Abdul
Geng, Qifang
Shimatani, Kenichiro
Hogetsu, Taizo
[J]. JOURNAL OF PLANT RESEARCH, 2006, 119 (04) : 415 - 417
[7] MINISTRY OF THE ENVIRONMENT OF JAPAN, 2000, VASC PLANTS, V8
[8] Rozen S, 2000, Methods Mol Biol, V132, P365
[9] PHYLOGENETIC-RELATIONSHIPS IN CROSSOSTYLIS (RHIZOPHORACEAE) INFERRED FROM RESTRICTION SITE VARIATION OF CHLOROPLAST DNA
SETOGUCHI, H
OHBA, H
[J]. JOURNAL OF PLANT RESEARCH, 1995, 108 (1089) : 87 - 92
[10] Genetic structure of the critically endangered plant Tricyrtis ishiiana (Convallariaceae) in relict populations of Japan
Setoguchi, Hiroaki
Mitsui, Yuki
Ikeda, Hajime
Nomura, Naofumi
Tamura, Atsushi
[J]. CONSERVATION GENETICS, 2011, 12 (02) : 491 - 501

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