Antifungal activity of oligochitosans (short chain chitosans) against some Candida species and clinical isolates of Candida albicans: Molecular weight-activity relationship

被引:94
作者
Kulikov, Sergey N. [1 ]
Lisovskaya, Svetlana A. [1 ]
Zelenikhin, Pavel V. [2 ]
Bezrodnykh, Evgeniya A. [3 ]
Shakirova, Diana R. [2 ]
Blagodatskikh, Inesa V. [3 ]
Tikhonov, Vladimir E. [3 ]
机构
[1] Kazan Sci Res Inst Epidemiol & Microbiol, Kazan 420015, Russia
[2] Kazan Fed Univ, Kazan 420008, Russia
[3] Russian Acad Sci, AN Nesmeyanov Inst Organoelement Cpds, Moscow 119991, Russia
关键词
Chitosan; Oligochitosan; Activity; Candida; Hyphae; TEM; Cytometry; POTENTIAL APPLICATIONS; ESCHERICHIA-COLI; DRUG-RESISTANCE; CHITIN; SUSCEPTIBILITY; DEPOLYMERIZATION; MODE; CHITIN/CHITOSAN; ANTIBIOTICS; COMBINATION;
D O I
10.1016/j.ejmech.2013.12.017
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
A series of oligochitosans (short chain chitosans) prepared by acidic hydrolysis of chitosan and characterized by their molecular weight, polydispersity and degree of deacetylation were used to determine their anticandidal activities. This study has demonstrated that oligochitosans show a high fungistatic activity (MIC 8-512 mu g/ml) against Candida species and clinical isolates of Candida albicans, which are resistant to a series of classic antibiotics. Flow cytometry analysis showed that oligochitosan possessed a high fungicidal activity as well. For the first time it was shown that even sub-MIC oligochitosan concentration suppressed the formation of C. albicans hyphal structures, cause severe cell wall alterations, and altered internal cell structure. These results indicate that oligochitosan should be considered as a possible alternative/additive to known anti-yeast agents in pharmaceutical compositions. (C) 2014 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:169 / 178
页数:10
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