Y chromosomal STR analysis using Pyrosequencing technology

被引：5
作者：
Edlund, Hanna [1 ]
Allen, Marie [1 ]
机构：
[1] Uppsala Univ, Dept Genet & Pathol, Rudbeck Lab, S-75185 Uppsala, Sweden
来源：
FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2009年 / 3卷 / 02期
关键词：
Pyrosequencing; Y chromosome STR; Forensic identification; SINGLE-NUCLEOTIDE POLYMORPHISM; FORENSIC CASEWORK; TANDEM REPEATS; DEGRADED DNA; HAPLOTYPES; AMPLIFICATION; MULTIPLEX; VALIDATION; IDENTIFICATION; PYROPHOSPHATE;
D O I：
10.1016/j.fsigen.2008.11.010
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Analysis of Y chromosome STIR markers has proven to be useful in forensic cases where the samples contain a mixture of DNA from several individuals. STR markers are commonly genotyped based on length separation of PCR products. In this study we evaluated if Pyrosequencing can be used as an alternative method for determining Y-STR variants. In total 70 unrelated Swedish males were typed for the Y chromosomal markers (DYS19, DYS389 I-II, DYS390, DYS391, DYS392, DYS393 and DYS438) using Pyrosequencing. Using the 8 markers, 57 unique haplotypes were observed with a discrimination capacity of 0.81. At four loci, the Pyrosequencing analysis revealed sequence variants. The sequence variants were found in the DYS389 11, DYS390, DYS391, and DYS393 loci in frequencies between 1.43% and 14.3%. Pyrosequencing has here been shown to be a useful tool for typing Y chromosomal STRs and the method can provide a complement to conventional forensic Y STIR analyses. Moreover, the Pyrosequencing method can be used to rapidly evaluate novel markers. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：119 / 124
页数：6
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