Electrochemical primer extension for the detection of single nucleotide polymorphisms in the cardiomyopathy associated MYH7 gene

被引：19
作者：
Debela, A. M. [1 ,2 ]
Thorimbert, S. [2 ]
Hasenknopf, B. [2 ]
O'Sullivan, C. K. [1 ,3 ]
Ortiz, M. [1 ]
机构：
[1] Univ Rovira & Virgili, Dept Engn Quim, E-43007 Tarragona, Spain
[2] Univ Paris 06, Inst Parisien Chim Mol, Univ Sorbonne, UMR CNRS 8232, F-75005 Paris, France
[3] Passeig Lluis Co 23, ICREA, Barcelona 08010, Spain
来源：
CHEMICAL COMMUNICATIONS | 2016年 / 52卷 / 04期
基金：
欧盟地平线“2020”;
关键词：
NUCLEOSIDE TRIPHOSPHATES; MUTATION DETECTION; HUMAN GENOME; REDOX LABEL; DNA; QUANTIFICATION; SEQUENCE; ARRAYS; PROBES; BASES;
D O I：
10.1039/c5cc07762a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We report the labelling of dideoxy nucleotides (ddNTPs) for use in electrochemical array based primer extension for the detection of single nucleotide polymorphisms (SNPs). The results confirm the extension of the immobilised primers for each of the four ddNTPs, representing a significant advance in achieving a cost-effective platform for screening of disease-specific SNPs.
引用
收藏
页码：757 / 759
页数：3
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