Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

被引:15
|
作者
Vojtech, Jennifer M. [1 ,2 ]
Cano-Mejia, Juliana [1 ,2 ]
Dumont, Matthieu F. [1 ]
Sze, Raymond W. [1 ,3 ]
Fernandes, Rohan [1 ,3 ,4 ]
机构
[1] Childrens Natl Med Ctr, Sheikh Zayed Inst Pediat Surg Innovat, Rockville, MD 20850 USA
[2] Univ Maryland, Fischell Dept Bioengn, College Pk, MD USA
[3] George Washington Univ, Dept Radiol, Washington, DC 20052 USA
[4] George Washington Univ, Dept Pediat, Washington, DC 20052 USA
来源
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2015年 / 98期
关键词
Bioengineering; Issue; 98; Prussian blue; nanoparticles; multimodal imaging; molecular imaging; fluorescence; magnetic resonance imaging; gadolinium; manganese; CONTRAST AGENTS;
D O I
10.3791/52621
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Multimodal, molecular imaging allows the visualization of biological processes at cellular, subcellular, and molecular-level resolutions using multiple, complementary imaging techniques. These imaging agents facilitate the real-time assessment of pathways and mechanisms in vivo, which enhance both diagnostic and therapeutic efficacy. This article presents the protocol for the synthesis of biofunctionalized Prussian blue nanoparticles (PB NPs) - a novel class of agents for use in multimodal, molecular imaging applications. The imaging modalities incorporated in the nanoparticles, fluorescence imaging and magnetic resonance imaging (MRI), have complementary features. The PB NPs possess a core-shell design where gadolinium and manganese ions incorporated within the interstitial spaces of the PB lattice generate MRI contrast, both in T-1 and T-2-weighted sequences. The PB NPs are coated with fluorescent avidin using electrostatic self-assembly, which enables fluorescence imaging. The avidin-coated nanoparticles are modified with biotinylated ligands that confer molecular targeting capabilities to the nanoparticles. The stability and toxicity of the nanoparticles are measured, as well as their MRI relaxivities. The multimodal, molecular imaging capabilities of these biofunctionalized PB NPs are then demonstrated by using them for fluorescence imaging and molecular MRI in vitro.
引用
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页数:14
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