Virulence genes in clinical and environmental Stenotrophomas maltophilia isolates: A genome sequencing and gene expression approach

被引：27

作者：

Adamek, Martina ^{[1
]}

Linke, Burkhard ^{[2
]}

Schwartz, Thomas ^{[1
]}

机构：

[1] Karlsruhe Inst Technol, Northern Campus, IFG, Microbiol Nat & Tech Interfaces Dept, D-76344 Eggenstein Leopoldshafen, Germany

[2] Univ Bielefeld, Ctr Biotechnol, D-33615 Bielefeld, Germany

来源：

MICROBIAL PATHOGENESIS | 2014年 / 67-68卷

关键词：

Stenotrophomonas maltophilia; Genome sequence; Virulence genes; RTX-toxin; RTX PROTEINS; INFECTIONS; DIVERSITY; BACTERIA; FAMILY; PILI;

D O I：

10.1016/j.micpath.2014.02.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The rate of nosocomial infections with the opportunistic pathogen Stenotrophomonas maltophilia has remarkably increased in the last decade. To determine S. maltophilia virulence genes, the complete genome sequences of two S. maltophilia isolates were compared. The clinical strain SKK35 was proved virulent in an amoeba host-pathogen model, and wastewater strain RA8 was determined as non-virulent in the amoeba model. The genome sequences of three additional S. maltophilia strains, K279a (clinical, non-virulent against amoeba), R511-3 and SKA14 (both environmental, non-virulent against amoeba) were taken into account as reference strains. We were able to show that all clinical and environmental S. maltophilia strains presented comparable distribution of so far identified potential virulence genes, regardless to their virulence potential against amoebae. Aside from that, strain SKK35 was found harboring a putative, strain specific pathogenicity island, encoding two proteins from the RTX (repeats-in-toxin) family. The actual expression of the RTX genes was verified in growth experiments in different culture media containing blood or blood components and in co-cultures with amoeba. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：20 / 30

页数：11

共 54 条

[41] High abundance of virulence gene homologues in marine bacteria [J].

Persson, Olof P. ;

Pinhassi, Jarone ;

Riemann, Lasse ;

Marklund, Britt-Inger ;

Rhen, Mikael ;

Normark, Staffan ;

Gonzalez, Jose M. ;

Hagstrom, Ake .

ENVIRONMENTAL MICROBIOLOGY, 2009, 11 (06) :1348-1357

[42] Adhesion to and biofilm formation on IB3-1 bronchial cells by Stenotrophomonas maltophilia isolates from cystic fibrosis patients [J].

Pompilio, Arianna ;

Crocetta, Valentina ;

Confalone, Pamela ;

Nicoletti, Mauro ;

Petrucca, Andrea ;

Guarnieri, Simone ;

Fiscarelli, Ersilia ;

Savini, Vincenzo ;

Piccolomini, Raffaele ;

Di Bonaventura, Giovanni .

BMC MICROBIOLOGY, 2010, 10

[43] Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli [J].

Ribitsch, D. ;

Heumann, S. ;

Karl, W. ;

Gerlach, J. ;

Leber, R. ;

Birner-Gruenberger, R. ;

Gruber, K. ;

Eiteljoerg, I. ;

Remler, P. ;

Siegert, P. ;

Lange, J. ;

Maurer, K. H. ;

Berg, G. ;

Guebitz, G. M. ;

Schwab, H. .

JOURNAL OF BIOTECHNOLOGY, 2012, 157 (01) :140-147

[44] Stenotrophomonas maltophilia strains replicate and persist in the murine lung, but to significantly different degrees [J].

Rouf, Ruella ;

Karaba, Sara M. ;

Dao, Jenny ;

Cianciotto, Nicholas P. .

MICROBIOLOGY-SGM, 2011, 157 :2133-2142

[45] The versatility and adaptation of bacteria from the genus Stenotrophomonas [J].

Ryan, Robert P. ;

Monchy, Sebastien ;

Cardinale, Massimiliano ;

Taghavi, Safiyh ;

Crossman, Lisa ;

Avison, Matthew B. ;

Berg, Gabriele ;

van der Lelie, Daniel ;

Dow, J. Maxwell .

NATURE REVIEWS MICROBIOLOGY, 2009, 7 (07) :514-525

[46] Pathogenicity islands in bacterial pathogenesis [J].

Schmidt, H ;

Hensel, M .

CLINICAL MICROBIOLOGY REVIEWS, 2004, 17 (01) :14-+

[47] Secretion of virulence determinants by the general secretory pathway in Gram-negative pathogens: an evolving story [J].

Stathopoulos, C ;

Hendrixson, DR ;

Thanassi, DG ;

Hultgren, SJ ;

St Geme, JW ;

Curtiss, R .

MICROBES AND INFECTION, 2000, 2 (09) :1061-1072

[48] Genome Survey and Characterization of Endophytic Bacteria Exhibiting a Beneficial Effect on Growth and Development of Poplar Trees [J].

Taghavi, Safiyh ;

Garafola, Craig ;

Monchy, Sebastien ;

Newman, Lee ;

Hoffman, Adam ;

Weyens, Nele ;

Barac, Tanja ;

Vangronsveld, Jaco ;

van der Lelie, Daniel .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2009, 75 (03) :748-757

[49] NEISSERIA-MENINGITIDIS PRODUCES IRON-REGULATED PROTEINS RELATED TO THE RTX FAMILY OF EXOPROTEINS [J].

THOMPSON, SA ;

WANG, LL ;

WEST, A ;

SPARLING, PF .

JOURNAL OF BACTERIOLOGY, 1993, 175 (03) :811-818

[50] Cable pili and the 22-kilodalton adhesin are required for Burkholderia cenocepacia binding to and transmigration across the squamous epithelium [J].

Urban, TA ;

Goldberg, JB ;

Forstner, JF ;

Sajjan, US .

INFECTION AND IMMUNITY, 2005, 73 (09) :5426-5437

← 1 2 3 4 5 6 →