Virulence genes in clinical and environmental Stenotrophomas maltophilia isolates: A genome sequencing and gene expression approach

被引：26
作者：
Adamek, Martina [1 ]
Linke, Burkhard [2 ]
Schwartz, Thomas [1 ]
机构：
[1] Karlsruhe Inst Technol, Northern Campus, IFG, Microbiol Nat & Tech Interfaces Dept, D-76344 Eggenstein Leopoldshafen, Germany
[2] Univ Bielefeld, Ctr Biotechnol, D-33615 Bielefeld, Germany
来源：
MICROBIAL PATHOGENESIS | 2014年 / 67-68卷
关键词：
Stenotrophomonas maltophilia; Genome sequence; Virulence genes; RTX-toxin; RTX PROTEINS; INFECTIONS; DIVERSITY; BACTERIA; FAMILY; PILI;
D O I：
10.1016/j.micpath.2014.02.001
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The rate of nosocomial infections with the opportunistic pathogen Stenotrophomonas maltophilia has remarkably increased in the last decade. To determine S. maltophilia virulence genes, the complete genome sequences of two S. maltophilia isolates were compared. The clinical strain SKK35 was proved virulent in an amoeba host-pathogen model, and wastewater strain RA8 was determined as non-virulent in the amoeba model. The genome sequences of three additional S. maltophilia strains, K279a (clinical, non-virulent against amoeba), R511-3 and SKA14 (both environmental, non-virulent against amoeba) were taken into account as reference strains. We were able to show that all clinical and environmental S. maltophilia strains presented comparable distribution of so far identified potential virulence genes, regardless to their virulence potential against amoebae. Aside from that, strain SKK35 was found harboring a putative, strain specific pathogenicity island, encoding two proteins from the RTX (repeats-in-toxin) family. The actual expression of the RTX genes was verified in growth experiments in different culture media containing blood or blood components and in co-cultures with amoeba. (C) 2014 Elsevier Ltd. All rights reserved.
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收藏
页码：20 / 30
页数：11
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