Bioconversion of 3-cyanopyridine to Nicotinic Acid by a Thermotable Nitrilase

被引：0
|
作者：
Nigam, V. K.
Agarwal, A.
Sharma, M.
Ghosh, P. [1 ]
Choudhury, B. [2 ]
机构：
[1] Birla Inst Sci Res, Jaipur 302001, Rajasthan, India
[2] Indian Inst Technol, Fac Biol Sci, Roorkee 247667, Uttaranchal, India
来源：
RESEARCH JOURNAL OF BIOTECHNOLOGY | 2009年 / 4卷 / 01期
关键词：
Nitrilase; 3-Cyanopyridine; Bacillus; Thermophilic; Biotransformation; RHODOCOCCUS-RHODOCHROUS J1; CATALYZED PRODUCTION; DEEP-SEA; PURIFICATION; ARYLACETONITRILASE; MANDELONITRILE; BIOCHEMISTRY; HYDROLYSIS; AMIDASE;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A thermostable nitrilase producing isolate identified as Bacillus sp. MTCC 7545 has been studied towards production of nicotinic acid from 3-cyanopyridine. The benzonitrile was observed as inducer for the enzyme production. Maximum enzyme production was observed at 24 h of incubation and the activity of nitrilase was found to be optimum at 65 degrees C. The enzyme was stable for similar to 6 h at 50 degrees C. Besides pyridine-3 carbonitrile, benzonitrile was also a preferred substrate for the enzyme.
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页码：32 / 36
页数：5
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