ATP synthesis in the disk membranes of rod outer segments of bovine retina

被引：5

作者：

Pepe, IM

Notari, L

Cugnoli, C

Panfoli, I

Morelli, A

机构：

[1] Univ Genoa, Fac Med, Ist Biofis, I-16132 Genoa, Italy

[2] Univ Genoa, Ist Chim Biol, I-16132 Genoa, Italy

[3] CNR, Ist Cibernet & Biofis, I-16146 Genoa, Italy

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2002年 / 66卷 / 02期

关键词：

Ca2+-ATPase; photoreception; vision energetics; disk membranes; bioenergetics;

D O I：

10.1016/S1011-1344(02)00238-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP is synthesized on the disk membrane isolated from rod outer segments of the bovine retina. Together with a slow component which accounted for a constant rate of about 22 nmol ATP/min/mg of protein and which was due to the adenylate kinase activity, a fast component with a maximal activity of about 58 nmol ATP/min/mg of protein was measured at physiological calcium concentrations. This fast activity disappeared in the presence of the Ca2+ ionophore A23187, was inhibited by vanadate or thapsigargin but not by oligomycin, suggesting that this ATP synthesis is due to the reversal functioning of the Ca2+-ATPase previously found on the disk membranes. (C) 2002 Elsevier Science BY All rights reserved.

引用

页码：148 / 152

页数：5

共 26 条

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[2]

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[3] EVIDENCE FOR 2 FUNCTIONALLY DIFFERENT MEMBRANE-FRACTIONS IN BOVINE RETINAL ROD OUTER SEGMENTS [J].