RNA polymerase II structure: from core to functional complexes

被引：73
作者：
Cramer, P
机构：
[1] Univ Munich, Inst Biochem, D-81377 Munich, Germany
[2] Univ Munich, Gene Ctr, D-81377 Munich, Germany
来源：
CURRENT OPINION IN GENETICS & DEVELOPMENT | 2004年 / 14卷 / 02期
关键词：
D O I：
10.1016/j.gde.2004.01.003
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
New structural studies of RNA polymerase II (Pol II) complexes mark the beginning of a detailed mechanistic analysis of the eukaryotic mRNA transcription cycle. Crystallographic models of the complete Pol II, together with new biochemical and electron microscopic data, give insights into transcription initiation. The first X-ray analysis of a Pol II complex with a transcription factor, the elongation factor THS, supports the idea that the polymerase has a 'tunable' active site that switches between mRNA synthesis and cleavage. The new studies also show that domains of transcription factors can enter polymerase openings, to modulate function during transcription.
引用
收藏
页码：218 / 226
页数：9
相关论文
共 68 条
[1]   Architecture of initiation-competent 12-subunit RNA polymerase II [J].
Armache, KJ ;
Kettenberger, H ;
Cramer, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (12) :6964-6968
[2]   DELINEATION OF 2 FUNCTIONAL REGIONS OF TRANSCRIPTION FACTOR-TFIIB [J].
BARBERIS, A ;
MULLER, CW ;
HARRISON, SC ;
PTASHNE, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5628-5632
[3]   STRUCTURAL BASIS FOR THE 3'-5' EXONUCLEASE ACTIVITY OF ESCHERICHIA-COLI DNA-POLYMERASE-I - A 2 METAL-ION MECHANISM [J].
BEESE, LS ;
STEITZ, TA .
EMBO JOURNAL, 1991, 10 (01) :25-33
[4]   Localization of the yeast RNA polymerase I-specific subunits [J].
Bischler, N ;
Brino, L ;
Carles, C ;
Riva, M ;
Tschochner, H ;
Mallouh, V ;
Schultz, P .
EMBO JOURNAL, 2002, 21 (15) :4136-4144
[5]   Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes [J].
Bleichenbacher, M ;
Tan, S ;
Richmond, TJ .
JOURNAL OF MOLECULAR BIOLOGY, 2003, 332 (04) :783-793
[6]   FUNCTIONAL DOMAINS OF TRANSCRIPTION FACTOR-TFIIB [J].
BURATOWSKI, S ;
ZHOU, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5633-5637
[7]   Complete, 12-subunit RNA polymerase II at 4.1-Å resolution:: Implications for the initiation of transcription [J].
Bushnell, DA ;
Kornberg, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (12) :6969-6973
[8]   Structural basis of transcription:: α-Amanitin-RNA polymerase II cocrystal at 2.8 A resolution [J].
Bushnell, DA ;
Cramer, P ;
Kornberg, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (03) :1218-1222
[9]   The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination [J].
Chédin, S ;
Riva, M ;
Schultz, P ;
Sentenac, A ;
Carles, C .
GENES & DEVELOPMENT, 1998, 12 (24) :3857-3871
[10]   Binding of TFIIB to RNA polymerase II: Mapping the binding site for the TFIIB zinc ribbon domain within the preinitiation complex [J].
Chen, HT ;
Hahn, S .
MOLECULAR CELL, 2003, 12 (02) :437-447
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