Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification

被引：8
|
作者：
Nogueira, Gabriela Ferreira [1 ]
Pasqual, Moacir [2 ]
Scherwinski-Pereira, Jonny Everson [1 ]
机构：
[1] Embrapa Recursos Genet & Biotecnol, BR-70770917 Brasilia, DF, Brazil
[2] Univ Fed Lavras, Dept Agr, BR-37200000 Lavras, MG, Brazil
来源：
PESQUISA AGROPECUARIA BRASILEIRA | 2013年 / 48卷 / 11期
关键词：
Saccharum; in vitro conservation; PVS2; regeneration; PROTOCOL;
D O I：
10.1590/S0100-204X2013001100014
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet-vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.
引用
收藏
页码：1524 / 1527
页数：4
相关论文
共 50 条
  • [41] Vitrification cryo-foil method for shoot tip cryopreservation and virus eradication in apple
    Wang, Min-Rui
    Bao, Jun-Hua
    Ma, Xiao-Yan
    Yan, Zi-Han
    Cui, Zhen-Hua
    Zhu, Li-Ying
    Zhang, Dong
    Wang, Qiao-Chun
    CRYOBIOLOGY, 2024, 117
  • [42] SHOOT-TIP CRYOPRESERVATION BY DROPLET VITRIFICATION OF Byrsonima intermedia A. JUSS.: A WOODY TROPICAL AND MEDICINAL PLANT SPECIES FROM BRAZILIAN CERRADO
    Silva, Luciano Coutinho
    Paiva, Renato
    Swennen, Rony
    Andre, Edwige
    Panis, Bart
    CRYOLETTERS, 2013, 34 (04) : 338 - 348
  • [43] Cryopreservation of in vitro-grown shoot tips of Clinopodium odorum using aluminium cryo-plates
    Engelmann-Sylvestre, Isabelle
    Engelmann, Florent
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2015, 51 (02) : 185 - 191
  • [44] CRYOPRESERVATION OF SHOOT TIPS AND COTYLEDONS OF THE NORTH AMERICAN GINSENG (Panax quinquefolius L.)
    Uchendu, E. E.
    Brown, D. C. W.
    Saxena, P. K.
    CRYOLETTERS, 2011, 32 (06) : 463 - 472
  • [45] Influence of the cryopreservation technique, recovery medium and genotype on genetic stability of mint cryopreserved shoot tips
    Martin, Carmen
    Kremer, Carolina
    Gonzalez, Ivan
    Elena Gonzalez-Benito, Maria
    PLANT CELL TISSUE AND ORGAN CULTURE, 2015, 122 (01) : 185 - 195
  • [46] Gold nanoparticles affect the cryopreservation efficiency of in vitro-derived shoot tips of bleeding heart
    Kulus, Dariusz
    Tymoszuk, Alicja
    PLANT CELL TISSUE AND ORGAN CULTURE, 2021, 146 (02) : 297 - 311
  • [47] CRYOPRESERVATION OF IN VITRO GROWN SHOOT TIPS OF Diospyros kaki Thunb. USING DIFFERENT METHODS
    Niu Yanli
    Luo Zhengrong
    Zhang Yanfang
    Zhang Qinglin
    CRYOLETTERS, 2012, 33 (01) : 69 - 74
  • [48] Cryopreservation of in vitro-grown shoot tips of Cleome rosea Vahl (Cleomaceae) using the V cryo-plate technique
    Cordeiro, Livia da Silva
    Simoes-Gurgel, Claudia
    Albarello, Norma
    Engelmann, Florent
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 2015, 51 (06) : 688 - 695
  • [49] EFFECT OF VARIOUS PRECULTURE AND OSMOTIC DEHYDRATION CONDITIONS ON CRYOPRESERVATION EFFICIENCY AND MORPHOGENETIC RESPONSE OF CHRYSANTHEMUM SHOOT TIPS
    Kulus, Dariusz
    Serocka, Magdalena
    Mikula, Anna
    ACTA SCIENTIARUM POLONORUM-HORTORUM CULTUS, 2018, 17 (01): : 139 - 147
  • [50] Cryopreservation of Pelargonium shoot tips by encapsulation-dehydration:: Effects of sucrose concentration, dehydration duration and genotype
    Grapin, A
    Dumet, D
    Holota, H
    Dorion, N
    PLANT GENETIC RESOURCES: THE FABRIC OF HORTICULTURE'S FUTURE, 2003, (623): : 225 - 230
12345