Survival of sugarcane shoot tips after cryopreservation by droplet-vitrification

被引：8

作者：

Nogueira, Gabriela Ferreira ^{[1
]}

Pasqual, Moacir ^{[2
]}

Scherwinski-Pereira, Jonny Everson ^{[1
]}

机构：

[1] Embrapa Recursos Genet & Biotecnol, BR-70770917 Brasilia, DF, Brazil

[2] Univ Fed Lavras, Dept Agr, BR-37200000 Lavras, MG, Brazil

来源：

PESQUISA AGROPECUARIA BRASILEIRA | 2013年 / 48卷 / 11期

关键词：

Saccharum; in vitro conservation; PVS2; regeneration; PROTOCOL;

D O I：

10.1590/S0100-204X2013001100014

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The objective of this work was to evaluate the phytotoxicity of a plant vitrification solution (PVS2), and the survival of shoot tips of the sugarcane variety SP716949, after cryopreservation by droplet-vitrification. Shoot tips were precultured for 24 hours in MS medium containing 0.3 mol L-1 sucrose, and exposed to PVS2 for 0, 20 or 30 min. Shoot tips were then immersed in liquid nitrogen. Thawing was fast in concentrated sucrose solution (1.2 mol L-1). PVS2 is a nontoxic to shoot tips, which in turn are sensitive to liquid nitrogen. The best results occurred when shoot tips were maintained for up to 20 min in PVS2 solution, before freezing, with 20% survival.

引用

页码：1524 / 1527

页数：4

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