Phytoplasmas Associated with Grapevine Yellows Disease in Chile

被引：36

作者：

Gajardo, A. ^{[1
]}

Fiore, N. ^{[1
]}

Prodan, S. ^{[1
]}

Paltrinieri, S. ^{[2
]}

Botti, S. ^{[2
]}

Pino, A. M. ^{[1
]}

Zamorano, A. ^{[1
]}

Montealegre, J. ^{[1
]}

Bertaccini, A. ^{[2
]}

机构：

[1] Univ Chile, Fac Ciencias Agron, Santiago, Chile

[2] Alma Mater Studiorum Univ Bologna, Dipartimento Sci & Tecnol Agroambientali, Bologna, Italy

来源：

PLANT DISEASE | 2009年 / 93卷 / 08期

关键词：

16S RIBOSOMAL-RNA; WITCHES-BROOM; ASH-YELLOWS; 1ST REPORT; SUBGROUP; CLASSIFICATION; IDENTIFICATION; REPRESENTS; ALFALFA; HOST;

D O I：

10.1094/PDIS-93-8-0789

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

An extensive survey was performed from 2002 to 2006 to detect and identify phytoplasmas associated with Chilean grapevines. Nested polymerase chain reaction assays using phytoplasma universal primer pairs P1/P7 and R16F2n/R2 detected phytoplasmas in 34 out of the 94 samples tested (36%). Restriction fragment length polymorphism (RFLP) analyses. cloning, and sequencing allowed identification of phytoplasmas belonging to ribosomal subgroups 16SrI-B 16SrI-C, 16SrVII-A, and l6SrXII-A. The 16SrVII-A phytoplasma represents a new finding ill grapevines moreover, variability of the RFLP profile was observed in some of the 16SrXII-A phytoplasmas, indicating possible new ribosomal subgroups. Mixed phytoplasma infections and infections of phytoplasmas together with one or more viruses also occurred.

引用

页码：789 / 796

页数：8

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