Soft metal thiol chemistry is not involved in the transport of arsenite by the Ars pump

被引：15

作者：

Chen, YX ^{[1
]}

Dey, S ^{[1
]}

Rosen, BP ^{[1
]}

机构：

[1] WAYNE STATE UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOL,DETROIT,MI 48201

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 03期

关键词：

D O I：

10.1128/jb.178.3.911-913.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The single cysteine in the ArsB protein subunit of the arsenite resistance pump was changed to serine and alanine residues. Resistance in cells expressing the two mutant arsB genes was the same as in the wild type, and the serine substitution had no effect on the arsenite transport properties. These results eliminate possible thiol chemistry in translocation. Thus, the pump uses soft metal chemistry for metalloactivation and nonmetal chemistry for oxyanion transport.

引用

页码：911 / 913

页数：3

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