Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells

被引：10
作者：
Nakamura, Michi [1 ]
Nishida, Hidetaka [2 ]
Yoshizaki, Karin [2 ]
Akiyoshi, Hideo [2 ]
Hatoya, Shingo [1 ]
Sugiura, Kikuya [1 ]
Inaba, Toshio [1 ]
机构：
[1] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Dept Adv Pathobiol, 1-58 Rinku Ourai Kita, Izumisano, Osaka 5988531, Japan
[2] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Dept Adv Clin Med, 1-58 Rinku Ourai Kita, Izumisano, Osaka 5988531, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2020年 / 82卷 / 05期
基金：
日本学术振兴会;
关键词：
conditioned medium; dog; mesenchymal stromal cell; neural differentiation; SPINAL-CORD-INJURY; NEURONAL DIFFERENTIATION; CEREBROSPINAL-FLUID; TRANSPLANTATION; EXPRESSION; RECOVERY; CULTURES; BRAIN;
D O I：
10.1292/jvms.19-0141
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
We examined the paracrine action of canine mesenchymal stromal cells (MSCs) derived from bone marrow on the survival and differentiation of neural stem cells (NSCs) in vitro. MSCs were collected from the proximal end of the diaphysis of femur of healthy beagle dogs. The 70-80% confluent MSCs were re-fed with serum-free DMEM. The MSCs were incubated for 48 hr and the supernatant was collected as the conditioned medium (MSC-CM). The survival rate of NSCs in MSC-CM was significantly greater than in the medium without MSC-CM. The percentage of differentiated neurons and neurite length in MSC-CM was also significantly higher than in the medium without MSC-CM. These results suggested that canine MSC-CM promotes stem cell survival and neural differentiation of NSCs.
引用
收藏
页码：668 / 672
页数：5
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