Canine mesenchymal stromal cell-conditioned medium promotes survival and neurite outgrowth of neural stem cells

被引：10
作者：
Nakamura, Michi [1 ]
Nishida, Hidetaka [2 ]
Yoshizaki, Karin [2 ]
Akiyoshi, Hideo [2 ]
Hatoya, Shingo [1 ]
Sugiura, Kikuya [1 ]
Inaba, Toshio [1 ]
机构：
[1] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Dept Adv Pathobiol, 1-58 Rinku Ourai Kita, Izumisano, Osaka 5988531, Japan
[2] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Dept Adv Clin Med, 1-58 Rinku Ourai Kita, Izumisano, Osaka 5988531, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2020年 / 82卷 / 05期
基金：
日本学术振兴会;
关键词：
conditioned medium; dog; mesenchymal stromal cell; neural differentiation; SPINAL-CORD-INJURY; NEURONAL DIFFERENTIATION; CEREBROSPINAL-FLUID; TRANSPLANTATION; EXPRESSION; RECOVERY; CULTURES; BRAIN;
D O I：
10.1292/jvms.19-0141
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
We examined the paracrine action of canine mesenchymal stromal cells (MSCs) derived from bone marrow on the survival and differentiation of neural stem cells (NSCs) in vitro. MSCs were collected from the proximal end of the diaphysis of femur of healthy beagle dogs. The 70-80% confluent MSCs were re-fed with serum-free DMEM. The MSCs were incubated for 48 hr and the supernatant was collected as the conditioned medium (MSC-CM). The survival rate of NSCs in MSC-CM was significantly greater than in the medium without MSC-CM. The percentage of differentiated neurons and neurite length in MSC-CM was also significantly higher than in the medium without MSC-CM. These results suggested that canine MSC-CM promotes stem cell survival and neural differentiation of NSCs.
引用
收藏
页码：668 / 672
页数：5
相关论文
共 50 条
[21]   Cord Blood Mesenchymal Stromal Cell-Conditioned Medium Protects Endothelial Cells via STAT3 Signaling [J].
Bader, Andreas Matthaeus ;
Brodarac, Andreja ;
Klose, Kristin ;
Bieback, Karen ;
Choi, Yeong-Hoon ;
Kang, Kyung-Sun ;
Kurtz, Andreas ;
Stamm, Christof .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2014, 34 (03) :646-657
[22]   MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronal- like cells by Schwann cell-conditioned medium [J].
Wei, Zhi-Jian ;
Fan, Bao-You ;
Liu, Yang ;
Ding, Han ;
Tang, Hao-Shuai ;
Pan, Da-Yu ;
Shi, Ba-Xiao ;
Zheng, Peng-Yuan ;
Shi, Hong-Yu ;
Wu, Heng ;
Li, Ang ;
Feng, Shi-Qing .
NEURAL REGENERATION RESEARCH, 2019, 14 (08) :1462-1469
[23]   Electrophysiological Characterisation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Induced by Olfactory Ensheathing Cell-Conditioned Medium [J].
Zeng, Yu ;
Rong, Mingqiang ;
Liu, Yunsheng ;
Liu, Jingfang ;
Lu, Ming ;
Tao, Xiaoyu ;
Li, Zhenyan ;
Chen, Xin ;
Yang, Kui ;
Li, Chuntao ;
Liu, Zhixiong .
NEUROCHEMICAL RESEARCH, 2013, 38 (12) :2483-2489
[24]   Therapeutic effects of human adipose stem cell-conditioned medium on stroke [J].
Cho, Yu Jin ;
Song, Hyeon Suk ;
Bhang, SukHo ;
Lee, Saehyoung ;
Kang, Bong Gu ;
Lee, Jae Chul ;
An, Jaeyeol ;
Cha, Choong Ik ;
Nam, Do-Hyun ;
Kim, Byung Soo ;
Joo, Kyeung Min .
JOURNAL OF NEUROSCIENCE RESEARCH, 2012, 90 (09) :1794-1802
[25]   Intraparenchymal treatment with bone marrow mesenchymal stem cell-conditioned medium exerts neuroprotection following intracerebral hemorrhage [J].
Cui, Changmeng ;
Cui, Ying ;
Gao, Junling ;
Li, Ran ;
Jiang, Xiaohua ;
Tian, Yanxia ;
Wang, Kaijie ;
Cui, Jianzhong .
MOLECULAR MEDICINE REPORTS, 2017, 15 (04) :2374-2382
[26]   Differentiation of mesenchymal stem cells to germ-like cells under induction of Sertoli cell-conditioned medium and retinoic acid [J].
Maghami, R. Ghaem ;
Mirzapour, T. ;
Bayrami, A. .
ANDROLOGIA, 2018, 50 (03)
[27]   Endothelial progenitor cell-conditioned medium promotes angiogenesis and is neuroprotective after spinal cord injury [J].
Wang, Tao ;
Fang, Xiao ;
Yin, Zong-Sheng .
NEURAL REGENERATION RESEARCH, 2018, 13 (05) :887-895
[28]   Human mesenchymal stem cell-conditioned medium improves cardiac function following myocardial infarction [J].
Timmers, Leo ;
Lim, Sai Kiang ;
Hoefer, Imo E. ;
Arslan, Fatih ;
Lai, Ruenn Chai ;
van Oorschot, Angelique A. M. ;
Goumans, Marie Jose ;
Strijder, Chaylendra ;
Sze, Sui Kwan ;
Choo, Andree ;
Piek, Jan J. ;
Doevendans, Pieter A. ;
Pasterkamp, Gerard ;
de Kleijn, Dominique P. V. .
STEM CELL RESEARCH, 2011, 6 (03) :206-214
[29]   Mesenchymal Stem Cell-Conditioned Medium Promotes Functional Recovery Following Spinal Cord Injury: A Systematic Review and Meta-analysis [J].
Sarveazad, Arash ;
Toloui, Amirmohammad ;
Moarrefzadeh, Aida ;
Nafchi, Hanieh Ghasemian ;
Neishaboori, Arian Madani ;
Yousefifard, Mahmoud .
SPINE SURGERY AND RELATED RESEARCH, 2022, 6 (05) :433-442
[30]   Effect of Bone Marrow Derived Mesenchymal Stem Cell Conditioned Media for Neurite Outgrowth in Human Ntera-2 Neurons [J].
Oh, Jin Soo ;
Liu, Meng Lu ;
Jin, Hong Lian ;
An, Sung Su ;
Kim, Keung Nyun ;
Yoon, Do Heum ;
Ha, Yoon .
TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2009, 6 (4-11) :562-567
12345