Genome sequence of Plasmopara viticola and insight into the pathogenic mechanism

被引：70

作者：

Yin, Ling ^{[1
,2
]}

An, Yunhe ^{[1
,3
]}

Qu, Junjie ^{[2
]}

Li, Xinlong ^{[1
]}

Zhang, Yali ^{[1
]}

Dry, Ian ^{[4
]}

Wu, Huijuan ^{[3
]}

Lu, Jiang ^{[1
,5
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

[2] Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China

[3] Beijing Ctr Phys & Chem Anal, Beijing 100089, Peoples R China

[4] CSIRO Agr & Food, Wine Innovat West Bldg, Urrbrae, SA 5064, Australia

[5] Shanghai Jiao Tong Univ, Sch Agr & Biol, Ctr Viticulture & Enol, Shanghai 200024, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

DOWNY MILDEW; PHYTOPHTHORA-SOJAE; PLANT PATHOGEN; RXLR EFFECTOR; DE-NOVO; RNA-SEQ; GRAPEVINE; RESISTANCE; ADAPTATION; GENES;

D O I：

10.1038/srep46553

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Plasmopara viticola causes downy mildew disease of grapevine which is one of the most devastating diseases of viticulture worldwide. Here we report a 101.3 Mb whole genome sequence of P. viticola isolate 'JL-7-2' obtained by a combination of Illumina and PacBio sequencing technologies. The P. viticola genome contains 17,014 putative protein-coding genes and has similar to 26% repetitive sequences. A total of 1,301 putative secreted proteins, including 100 putative RXLR effectors and 90 CRN effectors were identified in this genome. In the secretome, 261 potential pathogenicity genes and 95 carbohydrate- active enzymes were predicted. Transcriptional analysis revealed that most of the RXLR effectors, pathogenicity genes and carbohydrate- active enzymes were significantly up- regulated during infection. Comparative genomic analysis revealed that P. viticola evolved independently from the Arabidopsis downy mildew pathogen Hyaloperonospora arabidopsidis. The availability of the P. viticola genome provides a valuable resource not only for comparative genomic analysis and evolutionary studies among oomycetes, but also enhance our knowledge on the mechanism of interactions between this biotrophic pathogen and its host.

引用

页数：12

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