A 65-year-old woman was hospitalized with pyrexia and lumbar back pain. Radiographs of her lumbar spine and MRI were performed on the day of admission and the results were considered normal. Five days after admission, a Tc-99m MDP bone scan was performed and showed nonspecific increased uptake at L2-L3. Planar and SPECT Ga-67 citrate scintigraphy showed increased tracer uptake at L2-L3. MRI confirmed osteomyelitis at this site. A Tc-99m ciprofloxacin scan was performed and also showed infection at L2-L3. Group B Streptococcus grew in blood cultures from the patient, who improved clinically after a prolonged course of antibiotics.