miR-202 Promotes Cell Apoptosis in Esophageal Squamous Cell Carcinoma by Targeting HSF2

被引：27

作者：

Meng, Xiangrui ^{[1
]}

Chen, Xiaoqi ^{[2
]}

Lu, Peng ^{[3
]}

Ma, Wang ^{[1
]}

Yue, Dongli ^{[1
]}

Song, Lijie ^{[1
]}

Fan, Qingxia ^{[1
]}

机构：

[1] Zhengzhou Univ, Dept Oncol, Affiliated Hosp 1, Jianshe East Rd 1, Zhengzhou 450000, Peoples R China

[2] Henan Univ TCM, Dept Digest Oncol, Affiliated Hosp 1, Zhengzhou, Peoples R China

[3] Peoples Hosp Zhengzhou, Dept Gastrointestinal Surg, Zhengzhou, Peoples R China

来源：

ONCOLOGY RESEARCH | 2017年 / 25卷 / 02期

关键词：

miR-202; Esophageal squamous cell carcinoma (ESCC); Apoptosis; HSF2; Hsp70; EXPRESSION; PROLIFERATION; CANCER; DOWNSTREAM; MICRORNAS; SIGNATURE; MIRNA;

D O I：

10.3727/096504016X14732772150541

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant cancers with high mortality around the world. However, the regulatory mechanism of ESCC carcinogenesis is not completely known. Here we demonstrate the novel role of miR-202 in regulating ESCC cell apoptosis. The analysis of data obtained from the GEO database showed that the expression of miR-202 is aberrantly decreased in tumor tissue from ESCC patients and cultured ESCC cell lines. After transfection with miR-202 mimic or inhibitor, the apoptotic capacity of ESCC cells was significantly increased by miR-202 overexpression but reduced by miR-202 repression. We then identified HSF2 as a direct target of miR-202 with the binding site on the 3'-UTR of HSF2 mRNA in ESCC cells. The apoptosis of ESCC cells induced by the miR-202 mimic could be repressed by HSF2 overexpression. Further studies indicated that HSF2 overexpression strongly upregulated the expression of Hsp70 at both the mRNA and protein levels. In addition, HSF2/Hsp70 suppressed ESCC cell apoptosis by preventing caspase 3 activation. In conclusion, miR-202 is a potential tumor suppressor in human ESCC and acts by regulating the apoptosis of ESCC cells by targeting HSF2, in which caspase 3 activation is involved. This might provide a novel therapeutic target for human ESCC.

引用

页码：215 / 223

页数：9

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