Differential contribution of the Fanconi anemia-related proteins to repair of several types of DNA damage in cultured silkworm cells

被引：1
作者：
Sugahara, Ryohei [1 ,2 ]
Mon, Hiroaki [1 ]
Lee, Jae Man [1 ]
Shiotsuki, Takahiro [2 ]
Kusakabe, Takahiro [1 ]
机构：
[1] Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Lab Silkworm Sci, Fukuoka 8128581, Japan
[2] Natl Inst Agrobiol Sci, Insect Growth Regulat Res Unit, Tsukuba, Ibaraki 3058634, Japan
来源：
FEBS LETTERS | 2014年 / 588卷 / 21期
基金：
日本学术振兴会;
关键词：
DNA repair; Fanconi pathway; BLM pathway; Homologous recombination; Hydroxyurea; Camptothecin; FANCD2; MONOUBIQUITINATION; SPECIES LACKING; REPLICATION; COMPLEX; PATHWAY; RECOMBINATION; INTERACTS; BRCA2;
D O I：
10.1016/j.febslet.2014.09.009
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The silkworm Fanconi anemia (FA) pathway is required for normal cellular resistance to mitomycin C (MMC) in silkworms, but little is known about the requirement for repair of other types of DNA damage. Here we report that silkworm cells deficient for FA proteins FancD2 and FancM exhibit normal sensitivities to hydroxyurea (HU) and camptothecin (CPT), although FancM-dependent FancD2 monoubiquitination is induced upon these treatments. Similar results were observed in cells depleted for Rmi1 and Mhf1, which interact with the FancM protein. We also found that Rad51-knockdown cells exhibited normal sensitivity to HU despite induction of double-strand breaks by HU treatment. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：3959 / 3963
页数：5
相关论文
共 36 条
[1]   ATR couples FANCD2 monoubiquitination to the DNA-damage response [J].
Andreassen, PR ;
D'Andrea, AD ;
Taniguchi, T .
GENES & DEVELOPMENT, 2004, 18 (16) :1958-1963
[2]   BLM Deficiency Is Not Associated with Sensitivity to Hydroxyurea-Induced Replication Stress [J].
Bennani-Belhaj, Kenza Lahkim ;
Buhagiar-Labarchede, Geraldine ;
Jmari, Nada ;
Onclercq-Delic, Rosine ;
Amor-Gueret, Mounira .
JOURNAL OF NUCLEIC ACIDS, 2010, 2010
[3]   Identification of FAAP24, a Fanconi anemia core complex protein that interacts with FANCM [J].
Ciccia, Alberto ;
Ling, Chen ;
Coulthard, Rachel ;
Yan, Zhijiang ;
Xue, Yutong ;
Meetei, Amom Ruhikanta ;
Laghmani, El Houari ;
Joenje, Hans ;
McDonald, Neil ;
de Winter, Johan P. ;
Wang, Weidong ;
West, Stephen C. .
MOLECULAR CELL, 2007, 25 (03) :331-343
[4]   The genetic and molecular basis of Fanconi anemia [J].
de Winter, Johan P. ;
Joenje, Hans .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2009, 668 (1-2) :11-19
[5]   FANCM Connects the Genome Instability Disorders Bloom's Syndrome and Fanconi Anemia [J].
Deans, Andrew J. ;
West, Stephen C. .
MOLECULAR CELL, 2009, 36 (06) :943-953
[6]   Camptothecin enhances the frequency of oligonucleotide-directed gene repair in mammalian cells by inducing DNA damage and activating homologous recombination [J].
Ferrara, L ;
Kmiec, EB .
NUCLEIC ACIDS RESEARCH, 2004, 32 (17) :5239-5248
[7]   Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability [J].
French, CA ;
Masson, JY ;
Griffin, CS ;
O'Regan, P ;
West, SC ;
Thacker, J .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (22) :19322-19330
[8]   Interaction of the fanconi anemia proteins and BRCA1 in a common pathway [J].
Garcia-Higuera, I ;
Taniguchi, T ;
Ganesan, S ;
Meyn, MS ;
Timmers, C ;
Hejna, J ;
Grompe, M ;
D'Andrea, AD .
MOLECULAR CELL, 2001, 7 (02) :249-262
[9]  
Girard C., 2014, NUCL ACIDS RES
[10]   The FANCM/FAAP24 Complex Is Required for the DNA Interstrand Crosslink-Induced Checkpoint Response [J].
Huang, Min ;
Kim, Jung Min ;
Shiotani, Bunsyo ;
Yang, Kailin ;
Zou, Lee ;
D'Andrea, Alan D. .
MOLECULAR CELL, 2010, 39 (02) :259-268
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