Crystallization and preliminary X-ray diffraction studies of ferredoxin reductase from Leptospira interrogans

被引:4
作者
Nascimento, Alessandro S.
Ferrarezi, Thiago
Catalano-Dupuy, Daniela L.
Ceccarelli, Eduardo A.
Polikarpov, Igor
机构
[1] Univ Sao Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
[2] Univ Nacl Rosario, CONICET, Fac Ciencias Bioquim & Farmaceut, Div Mol Biol,Inst Biol Mol & Celular Rosario, RA-2002 Rosario, Argentina
来源
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2006年 / 62卷
关键词
D O I
10.1107/S1744309106020136
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Ferredoxin-NADP(+) reductase (FNR) is an FAD-containing enzyme that catalyzes electron transfer between NADP(H) and ferredoxin. Here, results are reported of the recombinant expression, purification and crystallization of FNR from Leptospira interrogans, a parasitic bacterium of animals and humans. The L. interrogans FNR crystals belong to a primitive monoclinic space group and diffract to 2.4 angstrom resolution at a synchrotron source.
引用
收藏
页码:662 / 664
页数:3
相关论文
共 23 条
[1]  
[Anonymous], 1992, Protein Crystallogr
[2]   THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].
BAILEY, S .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763
[3]  
Brunger AT, 1998, ACTA CRYSTALLOGR D, V54, P905, DOI 10.1107/s0907444998003254
[4]   Open questions in ferredoxin-NADP+ reductase catalytic mechanism [J].
Carrillo, N ;
Ceccarelli, EA .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (09) :1900-1915
[5]  
CECCARELLI EA, 2004, BIOCHIM BIOPHYS ACTA, V168, P155
[6]  
Deng Z, 1999, NAT STRUCT BIOL, V6, P847
[7]   The three-dimensional structure of flavodoxin reductase from Escherichia coli at 1.7 angstrom resolution [J].
Ingelman, M ;
Bianchi, V ;
Eklund, H .
JOURNAL OF MOLECULAR BIOLOGY, 1997, 268 (01) :147-157
[8]   ATOMIC-STRUCTURE OF FERREDOXIN-NADP+ REDUCTASE - PROTOTYPE FOR A STRUCTURALLY NOVEL FLAVOENZYME FAMILY [J].
KARPLUS, PA ;
DANIELS, MJ ;
HERRIOTT, JR .
SCIENCE, 1991, 251 (4989) :60-66
[9]   Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP+ reductase [J].
Kurisu, G ;
Kusunoki, M ;
Katoh, E ;
Yamazaki, T ;
Teshima, K ;
Onda, Y ;
Kimata-Ariga, Y ;
Hase, T .
NATURE STRUCTURAL BIOLOGY, 2001, 8 (02) :117-121
[10]   A structural basis of Equisetum arvense ferredoxin isoform II producing an alternative electron transfer with ferredoxin-NADP+ reductase [J].
Kurisu, G ;
Nishiyama, D ;
Kusunoki, M ;
Fujikawa, S ;
Katoh, M ;
Hanke, GT ;
Hase, T ;
Teshima, K .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (03) :2275-2281