Oligosaccharide sequencing based on exo- and endoglycosidase digestion and liquid chromatographic analysis of the products

被引：28
作者：
Prime, S [1 ]
Dearnley, J [1 ]
Ventom, AM [1 ]
Parekh, RB [1 ]
Edge, CJ [1 ]
机构：
[1] OXFORD GLYCOSYST LTD, ABINGDON OX14 1RG, OXON, ENGLAND
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 720卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(95)00029-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Exo- and endoglycosidases are used to sequence oligosaccharides and give valuable information on the monosaccharide sequence, together with the anomericity, the stereochemistry, and in some cases, the substitution pattern of the monosaccharides. Both sequential and parallel methods of oligosaccharide sequencing are discussed.
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页码：263 / 274
页数：12
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