The efficacy of lipopolysaccharide mixed chloroform-killed cell (LPS-CKC) bacterin of Pasteurella piscicida on Yellowtail, Seriola quinqueradiata

被引：23
作者：
Kawakami, H
Shinohara, N
Fukuda, Y
Yamashita, H
Kihara, H
Sakai, M
机构：
[1] MIYAZAKI UNIV, FAC AGR, MIYAZAKI 88921, JAPAN
[2] EHIME PREFECTURAL EXPT FISHERIES STN, UWAJIMA, EHIME 79801, JAPAN
[3] EHIME COLL HLTH SCI, DEPT MED LAB TECHNOL, TOBE, EHIME 79111, JAPAN
[4] OITA INST MARINE & FISHERIES SCI, AQUACULTURE DIV, OITA 87926, JAPAN
[5] EHIME PREFECTURAL FISH DIS CONTROL CTR, UWAJIMA, EHIME 798, JAPAN
来源：
AQUACULTURE | 1997年 / 154卷 / 02期
关键词：
LPS-CKC vaccine; Yellowtail; Pasteurella piscicida duration; field testing;
D O I：
10.1016/S0044-8486(97)00044-6
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
The effectiveness of the lipopolysaccharide-mixed chloroform-killed cell (LPS-CKC) vaccine against pasteurellosis was examined in yellowtail, Seriola quinqueradiata. Vaccination stimulated the production of serum agglutinating antibodies against Pasteurella piscicida. Production of a superoxide anion, as determined by reduction of nitroblue tetrazolium (NET), was increased in head kidney leucocytes of vaccinated fish. Fish vaccinated with the LPS-CKC bacterin showed a relative percentage survival (RPS) of 60% or greater when challenged by the immersion method using three different live P. piscicida strains and the efficacy of this bacterin continued for at least 35 days after vaccination, In addition to providing protection against an artificial challenge, the LPS-CKC bacterin also provided protection against natural infection by P. piscicida. (C) 1997 Elsevier Science B.V.
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收藏
页码：95 / 105
页数：11
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