Molecular Characterization of DNA Double Strand Breaks with Tip-Enhanced Raman Scattering

被引:68
|
作者
Lipiec, Ewelina [1 ,2 ]
Sekine, Ryo [2 ,3 ]
Bielecki, Jakub [1 ]
Kwiatek, Wojciech M. [1 ]
Wood, Bayden R. [2 ]
机构
[1] PAN, Henryk Niewodniczanski Inst Nucl Phys, PL-31342 Krakow, Poland
[2] Monash Univ, Sch Chem, Ctr Biospect, Clayton, Vic 3800, Australia
[3] Univ S Australia, Ctr Environm Risk Assessment & Remediat, Adelaide, SA 5095, Australia
基金
澳大利亚研究理事会;
关键词
atomic force microscopy; DNA damage; double strand breaks; tip-enhanced Raman scattering; ATOMIC-FORCE MICROSCOPY; DAMAGE; NANOSCALE; ELECTRONS; SPECTROSCOPY; NUCLEOBASES; GAMMA-H2AX; SPECTRA; CELLS;
D O I
10.1002/anie.201307271
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
DNA double strand breaks (DSBs) are deadly lesions that can lead to genetic defects and cell apoptosis. Techniques that directly detect DNA DSBs include scanning electron microscopy, atomic force microscopy (AFM), and fluorescence based approaches. While these techniques can be used to identify DSBs they provide no information on the molecular events occurring at the break. Tip-enhanced Raman scattering (TERS) can provide molecular information from DNA at the nanoscale and in combination with AFM provides a new way to visualize and characterize the molecular structure of DSBs. DSBs result from cleavage at the 3'- and 5'-bonds of deoxyribose upon exposure to UVC radiation based on the observation of POH and methyl/methylene deformation modes enhanced in the TERS spectra. It is hypothesized that strand fragments are hydrogen-terminated at the lesion, indicating the action of free radicals during photon exposure.
引用
收藏
页码:169 / 172
页数:4
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