Co-expression in Helicobacter pylori of cagA and non-opsonic neutrophil activation enhances the association with peptic ulcer disease

被引:14
作者
Danielsson, D
Farmery, SM
Blomberg, B
Perry, S
Rautelin, H
Crabtree, JE
机构
[1] St James Univ Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England
[2] Orebro Med Ctr Hosp, Dept Clin Microbiol & Immunol, S-70185 Orebro, Sweden
[3] Orebro Med Ctr Hosp, Dept Internal Med, Div Gastroenterol, S-70185 Orebro, Sweden
[4] Univ Helsinki, Dept Bacteriol & Immunol, Helsinki, Finland
关键词
Helicobacter pylori; neutrophil; cagA;
D O I
10.1136/jcp.53.4.318
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Aims-To investigate the association of cagA positivity and non-opsonic neutrophil activation capacity in wild-type Helicobacter pylori strains with peptic ulcer disease or chronic gastritis only. Methods-Helicobacter pylori were isolated from antral biopsies of 53 consecutive patients with chronic antral gastritis, of whom 24 had peptic ulcer disease endoscopically. The presence of cagA, a marker for the cag pathogenicity island, was determined by polymerase chain reaction with specific oligonucleotide primers, and non-opsonic neutrophil activation capacity by luminol enhanced chemiluminescence. Results-The cagA gene was present in 39 of 53 (73.6%) strains, 20 of which (83.3%) were from the 24 patients with peptic ulcer disease and 19 (65.5%) from the 29 patients with chronic gastritis only. Nonopsonic neutrophil activation was found in 29 (54.7%) strains, 16 of which (66.7%) were from patients with peptic ulcer disease, and 13 (44.8%) from those with chronic gastritis. Non-opsonic neutrophil activation was found more frequently in cagA(+) than cagA(-) strains (59% v 42.9%). Whereas four of the 14 cagA(-) strains and eight of the 24 non-opsonic neutrophil activation negative strains were from patients with peptic ulcer disease, only two of 24 (8.3%) peptic ulcer disease strains expressed neither cagA nor nonopsonic neutrophil activation. The cagA gene and non-opsonic neutrophil activation capacity were co-expressed in 14 of 24 (58.3%) strains from patients with peptic ulcer disease, and in nine of 29 (31%) strains from individuals with chronic gastritis. Conclusions-Positivity for cagA and non-opsonic neutrophil activation occur independently in wild-type H pylori strains. However, co-expression of the two markers enhanced the prediction of peptic ulcer disease.
引用
收藏
页码:318 / 321
页数:4
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