Functional and quantitative proteomics using SILAC

被引：725
作者：
Mann, Matthias [1 ]
机构：
[1] Max Planck Inst Biochem, Dept Proteom & Signal Transduct, D-82152 Martinsried, Germany
来源：
NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2006年 / 7卷 / 12期
关键词：
D O I：
10.1038/nrm2067
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Researchers in many biological areas now routinely characterize proteins by mass spectrometry. Among the many formats for quantitative proteomics, stable-isotope labelling by amino acids in cell culture (SILAC) has emerged as a simple and powerful one. SILAC removes false positives in protein-interaction studies, reveals large-scale kinetics of proteomes and - as a quantitative phosphoproteomics technology - directly uncovers important points in the signalling pathways that control cellular decisions.
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收藏
页码：952 / 958
页数：8
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