Functional cloning using pFB retroviral cDNA expression libraries

被引：5
作者：
Felts, KA [1 ]
Chen, K [1 ]
Zaharee, K [1 ]
Sundar, L [1 ]
Limjoco, J [1 ]
Miller, A [1 ]
Vaillancourt, P [1 ]
机构：
[1] Appl Mol Evolut, San Diego, CA 92121 USA
来源：
MOLECULAR BIOTECHNOLOGY | 2002年 / 22卷 / 01期
关键词：
expression cloning; cDNA library; retroviral packaging cell; gene therapy;
D O I：
10.1385/MB:22:1:025
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Retroviral cDNA expression libraries allow the efficient introduction of complex cDNA libraries into virtually any mitotic cell type for screening based on gene function. The cDNA copy number per cell can be easily controlled by adjusting the multiplicity of infection, thus cell populations may be generated in which >90% of infected cells contain one to three cDNAs. We describe the isolation of two known oncogenes and one cell surface receptor from a human Burkitt's lymphoma (Daudi) cDNA library inserted into the high-titer retroviral vector pFB.
引用
收藏
页码：25 / 32
页数：8
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