The matrix metalloproteinase inhibitor BB-94 improves the strength of intestinal anastomoses in the rat

被引:41
作者
de Hingh, IHJT
Siemonsma, MA
de Man, BM
Lomme, RMLM
Hendriks, T
机构
[1] Radboud Univ Nijmegen Med Ctr, Dept Surg, NL-6500 HB Nijmegen, Netherlands
[2] Canisius Wilhelmina Ziekenhuis, Dept Surg, Nijmegen, Netherlands
关键词
anastomosis; batimastat (BB-94); intestine; matrix metalloproteinase; rat;
D O I
10.1007/s00384-002-0418-3
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background and aims: The strength of intestinal anastomoses is relatively low in the first days after operation, possibly as a result of localized degradation of the supporting matrix by enzymes from the matrix metalloproteinase (MMP) family. This study examined whether BB-94, a broad spectrum inhibitor of MMP activity, could enhance anastomotic strength. Materials and methods: Male Wistar rats received anastomoses in both ileum and colon. From the day before operation onwards, animals were treated daily with BB-94 intraperitoneally at a dose of 30 mg/kg or with saline only. Rats were killed 1, 3, or 7 days after operation, and anastomotic bursting pressure and breaking strength were measured. On day 3 anastomotic hydroxyproline levels were measured, and MMP (gelatinase) activity was analyzed by gelatin zymography. Results: BB-94 strongly enhanced wound strength, hut only on day 3. when it was at its lowest. Daily administration increased median colonic and ileal breaking strength by 27% and 108%. respectively; colonic and ileal bursting pressure were increased by 54% and 58%, respectively. MMP activities were significantly lowered in anastomotic extracts from the rats treated with BB-94, Conclusion: Administration of BB-94 enhances anastomotic strength. Specific inhibition of MMP activity should be investigated further as a means to preserve anastomotic integrity.
引用
收藏
页码:348 / 354
页数:7
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