Cross Talk with Hematopoietic Cells Regulates the Endothelial Progenitor Cell Differentiation of CD34 Positive Cells

被引：38

作者：

Kwon, Sang-Mo ^{[1
]}

Lee, Jun-Hee ^{[1
]}

Lee, Sang-Hun ^{[2
]}

Jung, Seok-Yun ^{[1
]}

Kim, Da-Yeon ^{[1
]}

Kang, Song-Hwa ^{[1
]}

Yoo, So-Young ^{[1
]}

Hong, Jong-Kyu ^{[1
]}

Park, Ji-Hye ^{[1
]}

Kim, Jung-Hee ^{[1
]}

Kim, Sung-Wook ^{[1
]}

Kim, Yeon-Ju ^{[1
]}

Lee, Sun-Jin ^{[1
]}

Kim, Hwi-Gon ^{[3
]}

Asahara, Takayuki ^{[4
]}

机构：

[1] Pusan Natl Univ, Sch Med, Med Res Inst, Dept Physiol,Lab Vasc Med & Stem Cell Biol, Yangsan, South Korea

[2] Soonchunhyang Univ, Seoul Hosp, Soonchunhyang Med Sci Res Inst, Seoul, South Korea

[3] Pusan Natl Univ, Sch Med, Dept Obstet & Gynecol, Yangsan, South Korea

[4] Tokai Univ, Sch Med, Dept Regenerat Med Sci, Isehara, Kanagawa 25911, Japan

来源：

PLOS ONE | 2014年 / 9卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

BONE-MARROW; THERAPEUTIC ANGIOGENESIS; POSTNATAL VASCULOGENESIS; CLONOGENIC-ASSAY; CD31(+) CELLS; TRANSPLANTATION; NEOVASCULARIZATION; IMPLANTATION; STEM; IDENTIFICATION;

D O I：

10.1371/journal.pone.0106310

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Introduction: Despite the crucial role of endothelial progenitor cells (EPCs) in vascular regeneration, the specific interactions between EPCs and hematopoietic cells remain unclear. Methods: In EPC colony forming assays, we first demonstrated that the formation of EPC colonies was drastically increased in the coculture of CD34(+) and CD34(-) cells, and determined the optimal concentrations of CD34(+) cells and CD34(-) cells for spindle-shaped EPC differentiation. Results: Functionally, the coculture of CD34(+) and CD34(-) cells resulted in a significant enhancement of adhesion, tube formation, and migration capacity compared with culture of CD34(+) cells alone. Furthermore, blood flow recovery and capillary formation were remarkably increased by the coculture of CD34(+) and CD34(-) cells in a murine hind-limb ischemia model. To elucidate further the role of hematopoietic cells in EPC differentiation, we isolated different populations of hematopoietic cells. T lymphocytes (CD3(+)) markedly accelerated the early EPC status of CD34(+) cells, while macrophages (CD11b(+)) or megakaryocytes (CD41(+)) specifically promoted large EPC colonies. Conclusion: Our results suggest that specific populations of hematopoietic cells play a role in the EPC differentiation of CD34(+) cells, a finding that may aid in the development of a novel cell therapy strategy to overcome the quantitative and qualitative limitations of EPC therapy.

引用

页数：12

共 41 条

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