机构:
Harvard Univ, Dept Phys, Cambridge, MA 02138 USAHarvard Univ, Dept Phys, Cambridge, MA 02138 USA
Amir, Ariel
[1
]
Babaeipour, Farinaz
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Fac Arts & Sci, Ctr Syst Biol, Cambridge, MA 02138 USA
Univ Calif San Diego, Dept Phys, San Diego, CA 92093 USA
Univ Calif San Diego, Mol Biol Sect, San Diego, CA 92093 USAHarvard Univ, Dept Phys, Cambridge, MA 02138 USA
Babaeipour, Farinaz
[2
,3
,4
]
McIntosh, Dustin B.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Dept Phys, San Diego, CA 92093 USA
Univ Calif San Diego, Mol Biol Sect, San Diego, CA 92093 USAHarvard Univ, Dept Phys, Cambridge, MA 02138 USA
McIntosh, Dustin B.
[3
,4
]
Nelson, David R.
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Dept Phys, Cambridge, MA 02138 USA
Harvard Univ, Fac Arts & Sci, Ctr Syst Biol, Cambridge, MA 02138 USAHarvard Univ, Dept Phys, Cambridge, MA 02138 USA
Nelson, David R.
[1
,2
]
Jun, Suckjoon
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Fac Arts & Sci, Ctr Syst Biol, Cambridge, MA 02138 USA
Univ Calif San Diego, Dept Phys, San Diego, CA 92093 USA
Univ Calif San Diego, Mol Biol Sect, San Diego, CA 92093 USAHarvard Univ, Dept Phys, Cambridge, MA 02138 USA
Jun, Suckjoon
[2
,3
,4
]
机构:
[1] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA
[2] Harvard Univ, Fac Arts & Sci, Ctr Syst Biol, Cambridge, MA 02138 USA
[3] Univ Calif San Diego, Dept Phys, San Diego, CA 92093 USA
[4] Univ Calif San Diego, Mol Biol Sect, San Diego, CA 92093 USA
Cell walls define a cell's shape in bacteria. The walls are rigid to resist large internal pressures, but remarkably plastic to adapt to a wide range of external forces and geometric constraints. Currently, it is unknown how bacteria maintain their shape. In this paper, we develop experimental and theoretical approaches and show that mechanical stresses regulate bacterial cell wall growth. By applying a precisely controllable hydrodynamic force to growing rod-shaped Escherichia coli and Bacillus subtilis cells, we demonstrate that the cells can exhibit two fundamentally different modes of deformation. The cells behave like elastic rods when subjected to transient forces, but deform plastically when significant cell wall synthesis occurs while the force is applied. The deformed cells always recover their shape. The experimental results are in quantitative agreement with the predictions of the theory of dislocation-mediated growth. In particular, we find that a single dimensionless parameter, which depends on a combination of independently measured physical properties of the cell, can describe the cell's responses under various experimental conditions. These findings provide insight into how living cells robustly maintain their shape under varying physical environments.
机构:
Univ Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, NetherlandsUniv Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands
den Blaauwen, Tanneke
;
de Pedro, Miguel A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, Madrid, SpainUniv Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands
de Pedro, Miguel A.
;
Nguyen-Disteche, Martine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Liege, Ctr Ingn Proteines, Inst Chim B6a, Sart Tilman Par Liege, BelgiumUniv Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands
Nguyen-Disteche, Martine
;
Ayala, Juan A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, Madrid, SpainUniv Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands
机构:
Univ Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, NetherlandsUniv Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands
den Blaauwen, Tanneke
;
de Pedro, Miguel A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, Madrid, SpainUniv Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands
de Pedro, Miguel A.
;
Nguyen-Disteche, Martine
论文数: 0引用数: 0
h-index: 0
机构:
Univ Liege, Ctr Ingn Proteines, Inst Chim B6a, Sart Tilman Par Liege, BelgiumUniv Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands
Nguyen-Disteche, Martine
;
Ayala, Juan A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Autonoma Madrid, CSIC, Ctr Biol Mol Severo Ochoa, Madrid, SpainUniv Amsterdam, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands