Selective fluorometry of cytochrome c using glutathione-capped CdTe quantum dots in weakly basic medium

被引：43

作者：

Cao, Ming ^{[1
]}

Cao, Chun ^{[1
]}

Liu, Meigui ^{[1
]}

Wang, Peng ^{[1
]}

Zhu, Changqing ^{[1
]}

机构：

[1] Anhui Normal Univ, Coll Chem & Mat Sci, Anhui Key Lab Chemobiosensing, Wuhu 241000, Peoples R China

来源：

MICROCHIMICA ACTA | 2009年 / 165卷 / 3-4期

关键词：

CdTe quantum dots; Cytochrome c; Fluorescence quenching; CAPILLARY-ELECTROPHORESIS; GOLD ELECTRODE; LIVE CELLS; FLUORESCENCE; LUMINESCENT; NANOCRYSTALS; SPECTROSCOPY; PROTEINS; WATER;

D O I：

10.1007/s00604-009-0140-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glutathione-capped CdTe quantum dots (GSH-CdTe QDs) were synthesized in aqueous medium. Their interactions with proteins, especially three heme-containing proteins, were investigated over a broad pH range. At 6.0 < pH < 8.0, the fluorescence of the GSH-CdTe QDs can be effectively quenched by cytochrome c (Cyt. c) and hemoglobin, respectively. At pH > 8.0, only cytochrome c quenched the fluorescence of the GSH-CdTe QDs, and no significant fluorescence changes were observed for hemoglobin or other proteins. Based on the distinct fluorescence response, a novel method has been developed for the selective determination of cytochrome c using GSH-CdTe QDs as the fluorescence probe at pH 9.0. Under optimum conditions, the linear range of the calibration curve is from 3.2 x 10(-8) to 2.4 x 10(-6) mol L-1 and the detection limit is 3.0 x 10(-9) mol L-1. The method has been applied to the determination of cytochrome c in three synthetic and real samples, and satisfactory results were obtained with QDs through selecting the proper pH value.

引用

页码：341 / 346

页数：6

共 32 条

[1] A new determining method of copper(III) ions at ng ml-1 levels based on quenching of the water-soluble nanocrystals fluorescence [J].

Bo, C ;

Ping, Z .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 381 (04) :986-992

[2] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[3] Study of the adsorption of cytochrome c on a gold nanoparticle - modified gold electrode by using cyclic voltammetry, electrochemical impedance spectroscopy and chronopotentiometry [J].

Ding, Xiaoqin ;

Yang, Meng ;

Hu, Jingbo ;

Li, Qilong ;

McDougall, Angus .

MICROCHIMICA ACTA, 2007, 158 (1-2) :65-71

[4] High-efficiency fluorescence quenching of conjugated polymers by proteins [J].

Fan, CH ;

Plaxco, KW ;

Heeger, AJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (20) :5642-5643

[5] Discrete nanostructures of quantum dots/Au with DNA [J].

Fu, AH ;

Micheel, CM ;

Cha, J ;

Chang, H ;

Yang, H ;

Alivisatos, AP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (35) :10832-10833

[6] Efficient phase transfer of luminescent thiol-capped nanocrystals: From water to nonpolar organic solvents [J].

Gaponik, N ;

Talapin, DV ;

Rogach, AL ;

Eychmuller, A ;

Weller, H .

NANO LETTERS, 2002, 2 (08) :803-806

[7] Peptide-coated CdS quantum dots for the optical detection of copper(II) and silver(I) [J].

Gattás-Asfura, KA ;

Leblanc, RM .

CHEMICAL COMMUNICATIONS, 2003, (21) :2684-2685

[8] Analysis of reduced and oxidized forms of cytochrome c by capillary electrophoresis and capillary electrophoresis-mass spectrometry [J].

He, T ;

Chandramouli, N ;

Fu, E ;

Wu, A ;

Wang, YK .

ANALYTICAL BIOCHEMISTRY, 1999, 271 (02) :189-192

[9] Long-term multiple color imaging of live cells using quantum dot bioconjugates [J].

Jaiswal, JK ;

Mattoussi, H ;

Mauro, JM ;

Simon, SM .

NATURE BIOTECHNOLOGY, 2003, 21 (01) :47-51

[10] Surface-modified CdSe quantum dots as luminescent probes for cyanide determination [J].

Jin, WJ ;

Costa-Fernández, JM ;

Pereiro, R ;

Sanz-Medel, A .

ANALYTICA CHIMICA ACTA, 2004, 522 (01) :1-8

← 1 2 3 4 →