Cell type-specific reciprocal regulation of HIF1A gene expression is dependent on 5′- and 3′-UTRs

被引：13

作者：

Yasuda, Motoaki ^{[1
]}

Hatanaka, Tomoyuki ^{[1
]}

Shirato, Hiroki ^{[2
]}

Nishioka, Takeshi ^{[3
]}

机构：

[1] Hokkaido Univ, Grad Sch Dent Med, Dept Oral Pathobiol, Kita Ku, Sapporo, Hokkaido 0608586, Japan

[2] Hokkaido Univ, Sch Med, Dept Radiat Med, Sapporo, Hokkaido 0608638, Japan

[3] Hokkaido Univ, Grad Sch Hlth Sci, Dept Biomed Sci & Engn, Kita Ku, Sapporo, Hokkaido 0600812, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 447卷 / 04期

关键词：

Hypoxia; HIF-1; alpha; UTR; Translation; Transcription; INFLAMMATORY BREAST-CANCER; HYPOXIA-INDUCIBLE FACTORS; HIF-1-ALPHA; BINDING; TRANSLATION; TWIST; RNA; PROGRESSION; INITIATION; HUR;

D O I：

10.1016/j.bbrc.2014.04.058

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, we demonstrated the reciprocal regulation of hypoxia-inducible factor 1 alpha (HIF1A) gene expression via untranslated region-(UTR) dependent mechanisms. A 151 nucleotide sequence found in the HIF1A 5'-UTR is sufficient for significant translational up-regulation. On the other hand, the 3'-UTR of HIF1A has been implicated in mRNA degradation. In the non-metastatic breast cancer cell line MCF7, the 3'-UTR-dependent down-regulatory machinery predominates over the 5'-UTR-dependent up-regulation of HIF1A. However, 5'-UTR-dependent up-regulation is dominant among metastatic cell lines (MDA-MB453, U87MG). It is therefore likely that the predominance of 5'-UTR-dependent translational enhancement of HIF1A is critical for the malignant phenotype of cancer cells. PTBP-1, but not HuR, is a candidate RNA binding protein for the translational control of HIF1A. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：638 / 643

页数：6

共 25 条

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