Arabidopsis calcium-dependent protein kinase AtCPK1 plays a positive role in salt/drought-stress response

被引：62
作者：
Huang, Kui [1 ]
Peng, Lu [1 ]
Liu, Yingying [1 ]
Yao, Rundong [1 ]
Liu, Zhibin [1 ]
Li, Xufeng [1 ]
Yang, Yi [1 ]
Wang, Jianmei [1 ]
机构：
[1] Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Bioresource & Ecoenvironm, Chengdu 610065, Sichuan, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 498卷 / 01期
基金：
中国国家自然科学基金;
关键词：
AtCPK1; Arabidopsis; CDPKs; Salt stress; Drought stress; ABSCISIC-ACID; OXIDATIVE STRESS; SALT-STRESS; TOLERANCE; HOMEOSTASIS; CELLS; GENE;
D O I：
10.1016/j.bbrc.2017.11.175
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The calcium-dependent protein kinases (CDPKs) play vital roles in plant response to various environmental stimuli. Here, we investigated the function of Arabidopsis AtCPK1 in response to salt and drought stress. The loss-of-function cpk1 mutant displayed hypersensitive to salt and drought stress, whereas overexpressing AtCPK1 in Arabidopsis plants significantly enhanced the resistance to salt or drought stress. The reduced or elevated tolerance of cpk1 mutant and AtCPK1-overexpressing lines was confirmed by the changes of proline, malondialdehyde (MDA) and H2O2. Real-time PCR analysis revealed that the expression of several stress-inducible genes (RD29A, COR15A, ZAT10, APX2) down-regulated in cpk1 mutant and up-regulated in AtCPK1-overexpressing plants. These results are likely to indicate that AtCPK1 positively regulates salt and drought stress in Arabidopsis. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码：92 / 98
页数：7
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