Polysaccharide from Mesona chinensis: Extraction optimization, physicochemical characterizations and antioxidant activities

被引：113
作者：
Lin, Lihua [1 ]
Xie, Jianhua [1 ]
Liu, Suchen [1 ]
Shen, Mingyue [1 ]
Tang, Wei [1 ]
Xie, Mingyong [1 ]
机构：
[1] Nanchang Univ, State Key Lab Food Sci & Technol, 235 Nanjing East Rd, Nanchang 330047, Jiangxi, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2017年 / 99卷
基金：
中国国家自然科学基金;
关键词：
Mesona chinensis; Polysaccharide; Physicochemical characterizations; Extraction; Antioxidant activities; ULTRASOUND-ASSISTED EXTRACTION; WATER-SOLUBLE POLYSACCHARIDE; CHEMICAL-COMPOSITION; STRUCTURAL-CHARACTERIZATION; CYCLOCARYA; GUM; L;
D O I：
10.1016/j.ijbiomac.2017.03.040
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The optimization of extraction conditions, physicochemical characterizations and antioxidant activities of polysaccharide from Mesona chinensis (MCP) were investigated. Optimal extraction conditions of MCP with the highest yield of 7.05 +/- 0.12% was obtained by response surface methodology (RSM). The physicochemical characterizations of MCP obtained at the optimal extraction conditions were detected by TU-1900 spectrophotometer, high performance gel permeation chromatography (HPGPC), high-performance anion exchange chromatography (HPAEC) and fourier transform infrared spectroscopy (FT-IR) as well, revealing that MCP was a heteropolysaccharide containing uronic acid (29.3 +/- 11.3%) and protein (10.41 +/- 0.8%), with an average molecular weight (Mw) of 1.45 x 10(6) Da, which mainly consisted of galactose (Gal) and glucose (Glc) in a molar ratio of 1.00:1.38. Furthermore, MCP exhibited considerable antioxidant potential on scavenging hydroxyl (53.87 +/- 0.44%), superoxide anion (58.42 +/- 11.17%) and DPPH radicals (55.59 +/- 0.69%). Our results indicated that MCP might be a good candidate for further investigations of functional foods. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：665 / 673
页数：9
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