Bacillus subtilis;
iron oxide nanoparticles;
rice plant;
UV;
XRD;
FTIR;
SEM;
GREEN SYNTHESIS;
GROWTH;
STRESS;
GERMINATION;
PHYTOTOXICITY;
THREAT;
L;
D O I:
10.3390/toxics9010002
中图分类号:
X [环境科学、安全科学];
学科分类号:
08 ;
0830 ;
摘要:
Arsenic (As) contamination has emerged as a serious public health concern worldwide because of its accumulation and mobility through the food chain. Therefore, the current study was planned to check the effect of Bacillus subtilis-synthesized iron oxide nano particles (Fe3O4 NP) on rice (Oryza Sativa L.) growth against arsenic stress (0, 5, 10 and 15 ppm). Iron oxide nanoparticles were extracellular synthesized from Bacillus subtilis with a desired shape and size. The formations of nanoparticles were differentiated through UV-Visible Spectroscopy, FTIR, XRD and SEM. The UV-Visible spectroscopy of Bacillus subtilis-synthesized nanoparticles showed that the iron oxide surface plasmon band occurs at 268 nm. FTIR results revealed that different functional groups (aldehyde, alkene, alcohol and phenol) were present on the surface of nanoparticles. The SEM image showed that particles were spherical in shape with an average size of 67.28 nm. Arsenic toxicity was observed in seed germination and young seedling stage. The arsenic application significantly reduced seed germination (35%), root and shoots length (1.25 and 2.00 cm), shoot/root ratio (0.289), fresh root and shoots weight (0.205 and 0.260 g), dry root and shoots weight (6.55 and 6.75 g), dry matter percentage of shoot (12.67) and root (14.91) as compared to control. Bacillus subtilis-synthesized Fe3O4 NPs treatments (5 ppm) remarkably increased the germination (65%), root and shoot length (2 and 3.45 cm), shoot/root ratio (1.24) fresh root and shoot weight (0.335 and 0.275 mg), dry root and shoot weight (11.75 and 10.6 mg) and dry matter percentage of shoot (10.40) and root (18.37). Results revealed that the application of Fe3O4 NPs alleviated the arsenic stress and enhanced the plant growth. This study suggests that Bacillus subtilus-synthesized iron oxide nanoparticles can be used as nano-adsorbents in reducing arsenic toxicity in rice plants.
机构:
Tsinghua Univ, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China
Shenzhen Honglue Res Inst Innovat Management, Shenzhen 518119, Peoples R ChinaTsinghua Univ, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China
Zhou, Shuang
Qian, Wei
论文数: 0引用数: 0
h-index: 0
机构:
Tsinghua Univ, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China
Tsinghua Univ, Sch Environm, Beijing 100084, Peoples R ChinaTsinghua Univ, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China
Qian, Wei
Ning, Zigong
论文数: 0引用数: 0
h-index: 0
机构:
Harbin Inst Technol, Sch Civil & Environm Engn, Shenzhen 518055, Peoples R ChinaTsinghua Univ, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China
Ning, Zigong
Zhu, Xiaoshan
论文数: 0引用数: 0
h-index: 0
机构:
Tsinghua Univ, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China
Southern Lab Ocean Sci & Engn, Zhuhai 519000, Peoples R ChinaTsinghua Univ, Shenzhen Int Grad Sch, Shenzhen 518055, Peoples R China
机构:
Russian Armenian Univ, Dept Med Biochem & Biotechnol, 123 H Emin Str, Yerevan 0051, ArmeniaRussian Armenian Univ, Dept Med Biochem & Biotechnol, 123 H Emin Str, Yerevan 0051, Armenia
Gabrielyan, L.
Hakobyan, L.
论文数: 0引用数: 0
h-index: 0
机构:
Yerevan State Univ, Res Inst Biol, Biol Fac, Yerevan, ArmeniaRussian Armenian Univ, Dept Med Biochem & Biotechnol, 123 H Emin Str, Yerevan 0051, Armenia
Hakobyan, L.
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机构:
Hovhannisyan, A.
Trchounian, A.
论文数: 0引用数: 0
h-index: 0
机构:
Russian Armenian Univ, Dept Med Biochem & Biotechnol, 123 H Emin Str, Yerevan 0051, ArmeniaRussian Armenian Univ, Dept Med Biochem & Biotechnol, 123 H Emin Str, Yerevan 0051, Armenia
机构:
Materials and Nuclear Research School, Nuclear Science and Technology Research Institute (NSTRI), TehranShefa Neuroscience Research Center, Khatam ol Anbia Hospital, Tehran
Aghazadeh M.
Doroudi T.
论文数: 0引用数: 0
h-index: 0
机构:
Shefa Neuroscience Research Center, Khatam ol Anbia Hospital, TehranShefa Neuroscience Research Center, Khatam ol Anbia Hospital, Tehran
Doroudi T.
论文数: 引用数:
h-index:
机构:
Ganjali M.R.
Kolivand P.H.
论文数: 0引用数: 0
h-index: 0
机构:
Shefa Neuroscience Research Center, Khatam ol Anbia Hospital, TehranShefa Neuroscience Research Center, Khatam ol Anbia Hospital, Tehran
机构:
Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
Shaanxi Normal Univ, Sch Chem & Mat Sci, Xian 710062, Shaanxi, Peoples R ChinaChinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
Zhang, Yi
Chen, Biao
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
Soochow Univ, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Peoples R ChinaChinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
Chen, Biao
Zhang, Liming
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R ChinaChinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
Zhang, Liming
Huang, Jie
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R ChinaChinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
Huang, Jie
Chen, Fenghua
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R ChinaChinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
Chen, Fenghua
Yang, Zupei
论文数: 0引用数: 0
h-index: 0
机构:
Shaanxi Normal Univ, Sch Chem & Mat Sci, Xian 710062, Shaanxi, Peoples R ChinaChinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
Yang, Zupei
Yao, Jianlin
论文数: 0引用数: 0
h-index: 0
机构:
Soochow Univ, Coll Chem Chem Engn & Mat Sci, Suzhou 215123, Peoples R ChinaChinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
Yao, Jianlin
Zhang, Zhijun
论文数: 0引用数: 0
h-index: 0
机构:
Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R ChinaChinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou 215123, Peoples R China
机构:
Hong Kong Polytech Univ, Inst Text & Clothing, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Inst Text & Clothing, Kowloon, Hong Kong, Peoples R China
He, Fuan
Fan, Jintu
论文数: 0引用数: 0
h-index: 0
机构:
Hong Kong Polytech Univ, Inst Text & Clothing, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Inst Text & Clothing, Kowloon, Hong Kong, Peoples R China
Fan, Jintu
Ma, Dong
论文数: 0引用数: 0
h-index: 0
机构:
Sun Yat Sen Zhongshan Univ, Sch Chem & Chem Engn, Key Lab Designed Synth & Applicat Polymer Mat, Key Lab Polymer Composite & Funct Mat,Minist Educ, Guangzhou 510275, Guangdong, Peoples R ChinaHong Kong Polytech Univ, Inst Text & Clothing, Kowloon, Hong Kong, Peoples R China
Ma, Dong
Zhang, Liming
论文数: 0引用数: 0
h-index: 0
机构:
Sun Yat Sen Zhongshan Univ, Sch Chem & Chem Engn, Key Lab Designed Synth & Applicat Polymer Mat, Key Lab Polymer Composite & Funct Mat,Minist Educ, Guangzhou 510275, Guangdong, Peoples R ChinaHong Kong Polytech Univ, Inst Text & Clothing, Kowloon, Hong Kong, Peoples R China
Zhang, Liming
Leung, Chiwah
论文数: 0引用数: 0
h-index: 0
机构:
Hong Kong Polytech Univ, Dept Appl Phys, Kowloon, Hong Kong, Peoples R China
Hong Kong Polytech Univ, Mat Res Ctr, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Inst Text & Clothing, Kowloon, Hong Kong, Peoples R China
Leung, Chiwah
Chan, Helen Laiwa
论文数: 0引用数: 0
h-index: 0
机构:
Hong Kong Polytech Univ, Dept Appl Phys, Kowloon, Hong Kong, Peoples R China
Hong Kong Polytech Univ, Mat Res Ctr, Kowloon, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Inst Text & Clothing, Kowloon, Hong Kong, Peoples R China