Bacillus subtilis;
iron oxide nanoparticles;
rice plant;
UV;
XRD;
FTIR;
SEM;
GREEN SYNTHESIS;
GROWTH;
STRESS;
GERMINATION;
PHYTOTOXICITY;
THREAT;
L;
D O I:
10.3390/toxics9010002
中图分类号:
X [环境科学、安全科学];
学科分类号:
08 ;
0830 ;
摘要:
Arsenic (As) contamination has emerged as a serious public health concern worldwide because of its accumulation and mobility through the food chain. Therefore, the current study was planned to check the effect of Bacillus subtilis-synthesized iron oxide nano particles (Fe3O4 NP) on rice (Oryza Sativa L.) growth against arsenic stress (0, 5, 10 and 15 ppm). Iron oxide nanoparticles were extracellular synthesized from Bacillus subtilis with a desired shape and size. The formations of nanoparticles were differentiated through UV-Visible Spectroscopy, FTIR, XRD and SEM. The UV-Visible spectroscopy of Bacillus subtilis-synthesized nanoparticles showed that the iron oxide surface plasmon band occurs at 268 nm. FTIR results revealed that different functional groups (aldehyde, alkene, alcohol and phenol) were present on the surface of nanoparticles. The SEM image showed that particles were spherical in shape with an average size of 67.28 nm. Arsenic toxicity was observed in seed germination and young seedling stage. The arsenic application significantly reduced seed germination (35%), root and shoots length (1.25 and 2.00 cm), shoot/root ratio (0.289), fresh root and shoots weight (0.205 and 0.260 g), dry root and shoots weight (6.55 and 6.75 g), dry matter percentage of shoot (12.67) and root (14.91) as compared to control. Bacillus subtilis-synthesized Fe3O4 NPs treatments (5 ppm) remarkably increased the germination (65%), root and shoot length (2 and 3.45 cm), shoot/root ratio (1.24) fresh root and shoot weight (0.335 and 0.275 mg), dry root and shoot weight (11.75 and 10.6 mg) and dry matter percentage of shoot (10.40) and root (18.37). Results revealed that the application of Fe3O4 NPs alleviated the arsenic stress and enhanced the plant growth. This study suggests that Bacillus subtilus-synthesized iron oxide nanoparticles can be used as nano-adsorbents in reducing arsenic toxicity in rice plants.
机构:
Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi ArabiaImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Tombuloglu, Huseyin
Ercan, Ismail
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机构:
Duzce Univ, Fac Engn, Dept Elect & Elect Engn, TR-81010 Duzce, TurkiyeImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Ercan, Ismail
Alqahtani, Noha
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机构:
Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi ArabiaImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Alqahtani, Noha
Alotaibi, Bayan
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机构:
Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi ArabiaImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Alotaibi, Bayan
Bamhrez, Muruj
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Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi ArabiaImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Bamhrez, Muruj
Alshumrani, Raghdah
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机构:
Imam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi ArabiaImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Alshumrani, Raghdah
Turumtay, Halbay
论文数: 0引用数: 0
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机构:
Joint BioEnergy Inst, Emeryville, CA 94608 USA
Lawrence Berkeley Natl Lab, Environm Genom & Syst Biol Div, Berkeley, CA 94720 USA
Karadeniz Tech Univ, Dept Energy Syst Engn, TR-61830 Trabzon, TurkiyeImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Turumtay, Halbay
Ergin, Ibrahim
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机构:
Cukurova Univ, Fac Art & Sci, Dept Phys, TR-01330 Balcali Adana, TurkiyeImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Ergin, Ibrahim
Demirci, Tuna
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机构:
Duzce Univ, Sci & Technol Res Lab, TR-81560 Duzce, TurkiyeImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Demirci, Tuna
Ozcelik, Sezen
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机构:
Hakkari Univ, Fac Engn, Dept Food Engn, TR-30000 Hakkari, TurkiyeImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Ozcelik, Sezen
Kayed, Tarek Said
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机构:
Imam Abdulrahman Bin Faisal Univ, Coll Engn, Dept Basic Engn Sci, Dammam, Saudi ArabiaImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
Kayed, Tarek Said
Ercan, Filiz
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机构:
Imam Abdulrahman Bin Faisal Univ, Basic & Appl Sci Res Ctr, POB 1982, Dammam 31441, Saudi Arabia
Imam Abdulrahman Bin Faisal Univ, Coll Sci, Dept Phys, POB 1982, Dammam 31441, Saudi ArabiaImam Abdulrahman Bin Faisal Univ, Inst Res & Med Consultat IRMC, Dept Genet Res, POB 1982, Dammam 34221, Saudi Arabia
机构:
Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R ChinaNorthwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R China
Sainao, Wangqing
Shi, Zhenzhen
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机构:
Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R ChinaNorthwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R China
Shi, Zhenzhen
Pang, Hailong
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机构:
Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R ChinaNorthwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R China
Pang, Hailong
Feng, Hanqing
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机构:
Northwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R China
Northwest Normal Univ, New Rural Dev Res Inst, Lanzhou 730070, Gansu, Peoples R ChinaNorthwest Normal Univ, Coll Life Sci, Lanzhou 730070, Gansu, Peoples R China