The efficiency of a novel delivery system (PEI600-Tat) in development of potent DNA vaccine using HPV16 E7 as a model antigen

被引：29
作者：
Bolhassani, Azam [1 ,2 ]
Ghasemi, Nahid [2 ]
Servis, Catherine [3 ]
Taghikhani, Mohammad [2 ]
Rafati, Sima [1 ]
机构：
[1] Pasteur Inst Iran, Mol Immunol & Vaccine Res Lab, Tehran, Iran
[2] Tarbiat Modares Univ, Dept Clin Biochem, Tehran, Iran
[3] Univ Lausanne, Inst Biochem, CH-1066 Epalinges, Switzerland
来源：
DRUG DELIVERY | 2009年 / 16卷 / 04期
关键词：
Human papillomavirus (HPV); E7; delivery system; tat protein; polyethylenimine (PEI); DNA vaccination; CELL-PENETRATING PEPTIDES; GENE DELIVERY; TAT PEPTIDE; ENHANCED IMMUNOGENICITY; PROTEIN TRANSDUCTION; CATIONIC PEPTIDES; MAMMALIAN-CELLS; PLASMID DNA; CANCER; COMPLEXES;
D O I：
10.1080/10717540902757721
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
DNA vaccination is a promising approach for inducing both humoral and cellular immune responses. The mode of plasmid DNA delivery is critical to make progress in DNA vaccination. Using human papillomavirus type 16 E7 as a model antigen, this study evaluated the effect of peptide-polymer hybrid including PEI600-Tat conjugate as a novel gene delivery system on the potency of antigen-specific immunity in mice model. At ratio of 10:50 PEI-Tat/E7DNA (w/w), both humoral and cellular immune responses were significantly enhanced as compared with E7DNA construct and induced Th1 response. Therefore, this new delivery system could have promising applications in gene therapy.
引用
收藏
页码：196 / 204
页数：9
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