Production of recombinant rotavirus VP6 from a suspension culture of transgenic tomato (Lycopersicon esculentum Mill.) cells

被引：12
|
作者：
Chung, IS [1 ]
Kim, CH
Kim, KI
Hong, SH
Park, JH
Kim, JK
Kim, WY
机构：
[1] Kyung Hee Univ, Dept Genet Engn, Suwon 449701, South Korea
[2] Myung Ji Univ, Dept Biol Sci & Biotechnol, Yongin 449728, South Korea
[3] Chung Ang Univ, Dept Med Microbiol, Seoul 156756, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 04期
关键词：
high aspect rotating-wall vessel; production; rotavirus VP6; transgenic tomato cells;
D O I：
10.1023/A:1005626000329
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Recombinant rotavirus VP6 expressed in transgenic tomato cells was found primarily in the intracellular fraction and had a molecular weight of 44 kDa. In a shake flask, transgenic tomato cells produced 0.33 mg recombinant VP6 l(-1) after 18 days of incubation. In a high aspect rotating-wall vessel designed by NASA to simulate microgravity, the transgenic cells produced up to 0.15 mg recombinant VP6 l(-1).
引用
收藏
页码：251 / 255
页数：5
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