Production of recombinant rotavirus VP6 from a suspension culture of transgenic tomato (Lycopersicon esculentum Mill.) cells

被引：12

作者：

Chung, IS ^{[1
]}

Kim, CH

Kim, KI

Hong, SH

Park, JH

Kim, JK

Kim, WY

机构：

[1] Kyung Hee Univ, Dept Genet Engn, Suwon 449701, South Korea

[2] Myung Ji Univ, Dept Biol Sci & Biotechnol, Yongin 449728, South Korea

[3] Chung Ang Univ, Dept Med Microbiol, Seoul 156756, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 04期

关键词：

high aspect rotating-wall vessel; production; rotavirus VP6; transgenic tomato cells;

D O I：

10.1023/A:1005626000329

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant rotavirus VP6 expressed in transgenic tomato cells was found primarily in the intracellular fraction and had a molecular weight of 44 kDa. In a shake flask, transgenic tomato cells produced 0.33 mg recombinant VP6 l(-1) after 18 days of incubation. In a high aspect rotating-wall vessel designed by NASA to simulate microgravity, the transgenic cells produced up to 0.15 mg recombinant VP6 l(-1).

引用

页码：251 / 255

页数：5

共 50 条

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