A Novel Bispecific Antibody, S-Fab, Induces Potent Cancer Cell Killing

被引：22

作者：

Li, Li ^{[1
,2
]}

He, Ping ^{[1
,2
]}

Zhou, Changhua ^{[1
,2
]}

Jing, Li ^{[1
,2
]}

Dong, Bin ^{[1
,2
]}

Chen, Siqi ^{[1
,2
]}

Zhang, Ning ^{[3
]}

Liu, Yawei ^{[4
]}

Miao, Ji ^{[1
,2
]}

Wang, Zhong ^{[1
,2
]}

Li, Qing ^{[1
,2
]}

机构：

[1] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Ctr Cellular & Struct Biol, Guangzhou 510006, Guangdong, Peoples R China

[3] Peking Univ, Canc Hosp, Beijing Inst Canc Res, Beijing 100871, Peoples R China

[4] Hlth Div Guard Bur, Gen Staff Dept PLA, Beijing, Peoples R China

来源：

JOURNAL OF IMMUNOTHERAPY | 2015年 / 38卷 / 09期

关键词：

bispecific antibody; single-domain antibody; carcinoembryonic antigen; CD3; bacterial expression; SINGLE-DOMAIN ANTIBODIES; CARCINOEMBRYONIC ANTIGEN; CYTOTOXICITY; LYMPHOCYTES; ACTIVATION; EXPRESSION; REGION; TUMORS;

D O I：

10.1097/CJI.0000000000000099

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Bispecific antibodies that engage immune cells to kill cancer cells have been actively studied in cancer immunotherapy. In this study, we present a novel bispecific format, S-Fab, fabricated by linking a single-domain anti-carcinoembryonic antigen VHH to a conventional anti-CD3 Fab. In contrast to most bispecific antibodies, the S-Fab bispecific antibody can be efficiently expressed and purified from bacteria. The purified S-Fab is stable in serum and is able to recruit T cells to drive potent cancer cell killing. In xenograft models, the S-Fab antibody suppresses tumor growth in the presence of human immune cells. Our study suggested that the bispecific S-Fab format can be applied to a wide range of immunotherapies.

引用

页码：350 / 356

页数：7

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