Theoretical and experimental study of the role of cell-cell dipole interaction in dielectrophoretic devices: application to polynomial electrodes

被引：18

作者：

Camarda, Massimo ^{[1
]}

Fisicaro, Giuseppe ^{[1
]}

Anzalone, Ruggero ^{[1
]}

Scalese, Silvia ^{[1
]}

Alberti, Alessandra ^{[1
]}

La Via, Francesco ^{[1
]}

La Magna, Antonino ^{[1
]}

Ballo, Andrea ^{[2
]}

Giustolisi, Gianluca ^{[2
]}

Minafra, Luigi ^{[3
]}

Cammarata, Francesco P. ^{[3
]}

Bravata, Valentina ^{[3
]}

Forte, Giusi I. ^{[3
]}

Russo, Giorgio ^{[3
]}

Gilardi, Maria C. ^{[3
]}

机构：

[1] CNR IMM Sez Catania, I-95121 Catania, Italy

[2] Univ Catania, Dipartimento Ingn Elettr Elettron & Informat, Catania, Italy

[3] Ist Bioimmagini & Fisiol Mol IBFM CNR LATO, Cefalu, Sicilia, Italy

来源：

BIOMEDICAL ENGINEERING ONLINE | 2014年 / 13卷

关键词：

NANOTUBES;

D O I：

10.1186/1475-925X-13-71

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Background: We aimed to investigate the effect of cell-cell dipole interactions in the equilibrium distributions in dielectrophoretic devices. Methods: We used a three dimensional coupled Monte Carlo-Poisson method to theoretically study the final distribution of a system of uncharged polarizable particles suspended in a static liquid medium under the action of an oscillating non-uniform electric field generated by polynomial electrodes. The simulated distributions have been compared with experimental ones observed in the case of MDA-MB-231 cells in the same operating conditions. Results: The real and simulated distributions are consistent. In both cases the cells distribution near the electrodes is dominated by cell-cell dipole interactions which generate long chains. Conclusions: The agreement between real and simulated cells' distributions demonstrate the method's reliability. The distribution are dominated by cell-cell dipole interactions even at low density regimes (10(5) cell/ml). An improved estimate for the density threshold governing the interaction free regime is suggested.

引用

页数：10

共 30 条

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