Intercellular communication in Arabidopsis thaliana pollen discovered via AHG3 transcript movement from the vegetative cell to sperm

被引：22

作者：

Jiang, Hua ^{[1
,2
,3
,4
]}

Yi, Jun ^{[1
,2
,3
,4
]}

Boavida, Leonor C. ^{[5
]}

Chen, Yuan ^{[1
,2
]}

Becker, Joerg D. ^{[5
]}

Kohler, Claudia ^{[3
,4
]}

McCormick, Sheila ^{[1
,2
]}

机构：

[1] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA

[2] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

[3] Swedish Univ Agr Sci, Dept Plant Biol, Uppsala BioCtr, S-75007 Uppsala, Sweden

[4] Linnean Ctr Plant Biol, S-75007 Uppsala, Sweden

[5] Inst Gulbenkian Ciencias, Plant Genom Lab, P-2780156 Oeiras, Portugal

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2015年 / 112卷 / 43期

基金：

欧洲研究理事会;

关键词：

pollen; sperm; mRNA transport; protein phosphatase 2C; vegetative nucleus; GENE-EXPRESSION; MESSENGER-RNA; PROTEIN-SYNTHESIS; TO-CELL; PLANTS; LOCALIZATION; IDENTIFICATION; TRANSFORMATION; FERTILIZATION; PROMOTERS;

D O I：

10.1073/pnas.1510854112

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An Arabidopsis pollen grain (male gametophyte) consists of three cells: the vegetative cell, which forms the pollen tube, and two sperm cells enclosed within the vegetative cell. It is still unclear if there is intercellular communication between the vegetative cell and the sperm cells. Here we show that ABA-hypersensitive germination3 (AHG3), encoding a protein phosphatase, is specifically transcribed in the vegetative cell but predominantly translated in sperm cells. We used a series of deletion constructs and promoter exchanges to document transport of AHG3 transcripts from the vegetative cell to sperm and showed that their transport requires sequences in both the 5' UTR and the coding region. Thus, in addition its known role in transporting sperm during pollen tube growth, the vegetative cell also contributes transcripts to the sperm cells.

引用

页码：13378 / 13383

页数：6

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