Methods to rapidly and accurately screen a large number of ENU mutagenized mice for abnormal motor phenotypes

被引:14
作者
Abramow-Newerly, Wanda
Lipina, Tatiana
Abramow-Newerly, Maria
Kim, Daniel
Bechard, Allison R.
Xie, Gang
Clapcote, Steve J.
Roder, John C.
机构
[1] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
[2] Univ Toronto, Dept Mol & Med Genet, Toronto, ON, Canada
来源
AMYOTROPHIC LATERAL SCLEROSIS | 2006年 / 7卷 / 02期
关键词
ENU mutagenesis; mice; motor impairment; SOD1-G93A;
D O I
10.1080/14660820500443000
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
In a dominant genetic screen for late-onset motor impairments in mice, 16-20-week-old N-nitroso-N-ethylurea (ENU)-mutagenized females were subjected to a behavioural test battery consisting of a visual assessment followed by the vertical pole, rotarod and grip strength tests. SOD1-G93A transgenic mice were tested in parallel as a positive control to provide information on the validity and sensitivity of the screen. From among the 1500 G(1) ENU mice screened, four affected mice with impaired motor function were classified as outliers. Approximately 32% of the G(2) and G(3) progeny of one outlier were affected. Vertical pole, rotarod and grip strength test scores were significantly correlated with each other and with body weight in the G(1) progeny, but the correlation with body weight was not maintained in the G(2) and G(3) progeny. We found that two tests, tail suspension and vertical pole, were sufficient to distinguish ENU outliers and SOD1-G93A hemizygotes from control mice, and could detect abnormalities earlier and more frequently than the other tests employed.
引用
收藏
页码:112 / 118
页数:7
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