Methods to rapidly and accurately screen a large number of ENU mutagenized mice for abnormal motor phenotypes
被引:14
作者:
Abramow-Newerly, Wanda
论文数: 0引用数: 0
h-index: 0
机构:Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
Abramow-Newerly, Wanda
Lipina, Tatiana
论文数: 0引用数: 0
h-index: 0
机构:Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
Lipina, Tatiana
Abramow-Newerly, Maria
论文数: 0引用数: 0
h-index: 0
机构:Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
Abramow-Newerly, Maria
Kim, Daniel
论文数: 0引用数: 0
h-index: 0
机构:Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
Kim, Daniel
Bechard, Allison R.
论文数: 0引用数: 0
h-index: 0
机构:Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
Bechard, Allison R.
Xie, Gang
论文数: 0引用数: 0
h-index: 0
机构:Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
Xie, Gang
Clapcote, Steve J.
论文数: 0引用数: 0
h-index: 0
机构:Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
Clapcote, Steve J.
Roder, John C.
论文数: 0引用数: 0
h-index: 0
机构:Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
Roder, John C.
机构:
[1] Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada
[2] Univ Toronto, Dept Mol & Med Genet, Toronto, ON, Canada
来源:
AMYOTROPHIC LATERAL SCLEROSIS
|
2006年
/
7卷
/
02期
关键词:
ENU mutagenesis;
mice;
motor impairment;
SOD1-G93A;
D O I:
10.1080/14660820500443000
中图分类号:
R74 [神经病学与精神病学];
学科分类号:
摘要:
In a dominant genetic screen for late-onset motor impairments in mice, 16-20-week-old N-nitroso-N-ethylurea (ENU)-mutagenized females were subjected to a behavioural test battery consisting of a visual assessment followed by the vertical pole, rotarod and grip strength tests. SOD1-G93A transgenic mice were tested in parallel as a positive control to provide information on the validity and sensitivity of the screen. From among the 1500 G(1) ENU mice screened, four affected mice with impaired motor function were classified as outliers. Approximately 32% of the G(2) and G(3) progeny of one outlier were affected. Vertical pole, rotarod and grip strength test scores were significantly correlated with each other and with body weight in the G(1) progeny, but the correlation with body weight was not maintained in the G(2) and G(3) progeny. We found that two tests, tail suspension and vertical pole, were sufficient to distinguish ENU outliers and SOD1-G93A hemizygotes from control mice, and could detect abnormalities earlier and more frequently than the other tests employed.
机构:
Wake Forest Univ, Sch Med, Comparat Med Clin Res Ctr, Winston Salem, NC 27157 USAWake Forest Univ, Sch Med, Comparat Med Clin Res Ctr, Winston Salem, NC 27157 USA
Clarkson, TB
Appt, SE
论文数: 0引用数: 0
h-index: 0
机构:
Wake Forest Univ, Sch Med, Comparat Med Clin Res Ctr, Winston Salem, NC 27157 USAWake Forest Univ, Sch Med, Comparat Med Clin Res Ctr, Winston Salem, NC 27157 USA
机构:
Wake Forest Univ, Sch Med, Comparat Med Clin Res Ctr, Winston Salem, NC 27157 USAWake Forest Univ, Sch Med, Comparat Med Clin Res Ctr, Winston Salem, NC 27157 USA
Clarkson, TB
Appt, SE
论文数: 0引用数: 0
h-index: 0
机构:
Wake Forest Univ, Sch Med, Comparat Med Clin Res Ctr, Winston Salem, NC 27157 USAWake Forest Univ, Sch Med, Comparat Med Clin Res Ctr, Winston Salem, NC 27157 USA