Ace-enhanced laser desorption/lonization-time of flight-mass spectrometry (SELDI-TOF-MS): A new proteomic urinary test for patients with urolithiasis

被引：47

作者：

Cadieux, PA

Beiko, DT

Watterson, JD

Burton, JP

Howard, JC

Knudsen, BE

Gan, BS

McCormick, JK

Chambers, AF

Denstedt, JD

Reid, G

机构：

[1] Lawson Hlth Res Inst, Canadian Res & Dev Ctr Probiot, London, ON N6A 4V2, Canada

[2] Univ Western Ontario, Dept Microbiol & Immunol, London, ON, Canada

[3] Univ Western Ontario, Dept Oncol, London, ON, Canada

[4] Univ Western Ontario, Dept Biochem, London, ON, Canada

[5] Univ Western Ontario, Dept Physiol & Pharmacol, London, ON, Canada

[6] Univ Western Ontario, Dept Med Biophys, London, ON, Canada

[7] Univ Western Ontario, Dept Urol, London, ON, Canada

[8] Univ Western Ontario, Div Plast Surg, London, ON, Canada

[9] Univ Western Ontario, Dept Surg, London, ON, Canada

来源：

JOURNAL OF CLINICAL LABORATORY ANALYSIS | 2004年 / 18卷 / 03期

关键词：

urinary tract calculi; albumin; protein profiling; diagnostics; urine;

D O I：

10.1002/jcla.20018

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

SELDI-TOF-MS is a highly sensitive protein-analysis tool capable of detecting minute protein profile differences between biological samples. As proteins have been associated with urinary tract calculi, protein-based urinalysis may offer insights into their diagnosis. The purpose of this study was to evaluate SELDI-TOF-MS as a potential method for identifying urinary biomarkers of urolithiasis. Midstream sterile urine samples were obtained from 25 male patients with a confirmed diagnosis of urolithiasis (test group) and 25 male subjects with no known history of the disease (controls). Urinary levels of oxalate, total protein, albumin, and osteopontin were determined. Protein profiles were generated using SELDI-TOF-MS. SELDI-TOF-MS profiling revealed a relationship between protein peak intensities at 67 and 24 kDa that differed between the two groups. The ratio of p67:p24 was found to be less than 1.0 in all of the control samples (mean 0.26), while 18 out of 25 (72%) of the test group samples displayed a ratio greater than 1.0 (total group mean 4.75, P<0.001). Albumin, total protein, and oxalate levels were higher in the test group than the controls. Although SELDI-TOF-MS is not yet in widespread use in hospital and diagnostic laboratories, this system represents a promising new method for rapidly identifying patients with urolithiasis. (c) 2004 WileyLiss, Inc.

引用

页码：170 / 175

页数：6

共 17 条

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