Field evaluation of a novel colorimetric method -: Double-site enzyme-linked lactate dehydrogenase immunodetection assay -: To determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand

被引：15
作者：
Brockman, A
Singlam, S
Phiaphun, L
Looareesuwan, S
White, NJ
Nosten, F
机构：
[1] Shoklo Malaria Res Unit, Tak 63110, Thailand
[2] Mahidol Univ, Fac Trop Med, Bangkok 10400, Thailand
[3] Univ Queensland, Australian Ctr Int Trop Hlth & Nutr, Brisbane, Qld, Australia
[4] John Radcliffe Hosp, Nuffield Dept Clin Med, Ctr Trop Dis, Oxford, England
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 04期
关键词：
D O I：
10.1128/AAC.48.4.1426-1429.2004
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A double-site enzyme-linked lactate dehydrogenase enzyme inummodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/mul of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.
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页码：1426 / 1429
页数：4
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