Breast tumour cell-induced down-regulation of type I collagen mRNA in fibroblasts

被引:32
作者
Fenhalls, G
Geyp, M
Dent, DM
Parker, MI [1 ]
机构
[1] Univ Cape Town, Fac Hlth Sci, Dept Biochem Med, ZA-7925 Cape Town, South Africa
[2] Univ Cape Town, Fac Hlth Sci, Dept Surg, ZA-7925 Cape Town, South Africa
基金
英国医学研究理事会;
关键词
breast cancer; extracellular matrix; cell-cell interaction; collagen gene expression;
D O I
10.1038/sj.bjc.6690821
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
This study investigated the modulation of type I collagen gene expression in normal fibroblasts by breast tumour cells. Northern analysis of total RNA extracted from stages I, II and III breast tumour tissue revealed that collagen mRNA levels were elevated in stage I tumours compared to the adjacent normal breast tissues, whereas they were decreased in stages II and III breast tumours. This aberrant collagen gene expression was confirmed by non-radioactive RNA:RNA in situ hybridization analysis of 30 breast carcinomas which localized the production of type I collagen mRNA to the stromal fibroblasts within the vicinity of the tumour cells, In order to determine whether the tumour cells were directly responsible for this altered collagen production by the adjacent fibroblasts, breast tumour cell lines were co-cultured with normal fibroblasts for in vitro assessment of collagen and steady-state collagen RNA levels, Go-culture of tumour cells and normal fibroblasts in the same dish resulted in down-regulation of collagen mRNA and protein. Treatment of the fibroblasts with tumour-cell conditioned medium also resulted in decreased collagen protein levels but the mRNA levels, however, remained unaltered. These results suggested that the tumour cells either secrete a labile 'factor', or express a cell surface protein requiring direct contact with the fibroblasts, resulting in down-regulation of collagen gene expression. Modulation of the ECM is a common characteristic of invading tumour cells and usually involves increased production of collagenases by the tumour cells or stromal fibroblasts. This study showed that tumour cells were also able to modulate collagen mRNA production by stromal fibroblasts, which may facilitate tumour cell invasion and metastasis. (C) 1999 Cancer Research Campaign.
引用
收藏
页码:1142 / 1149
页数:8
相关论文
共 50 条
[41]   Sodium tanshinone IIA sulfonate attenuates angiotensin II-induced collagen type I expression in cardiac fibroblasts in vitro [J].
Yang, Le ;
Zou, Xiao-Jing ;
Gao, Xiang ;
Chen, Hao ;
Luo, Jin-Long ;
Wang, Zhao-Hua ;
Liang, Qian-Sheng ;
Yang, Guang-Tian .
EXPERIMENTAL AND MOLECULAR MEDICINE, 2009, 41 (07) :508-516
[42]   Lysophosphatidic acid inhibits TGF-β-mediated stimulation of type I collagen mRNA stability via an ERK-dependent pathway in dermal fibroblasts [J].
Sato, M ;
Shegogue, D ;
Hatamochi, A ;
Yamazaki, S ;
Trojanowska, M .
MATRIX BIOLOGY, 2004, 23 (06) :353-361
[43]   Down-regulation of RIP1 by 2-deoxy-D-glucose sensitizes breast cancer cells to TRAIL-induced apoptosis [J].
Huang, Ying Ying ;
Liu, Hao ;
Li, Yang ;
Pu, Long Jian ;
Jiang, Chen Chen ;
Xu, Jin Cheng ;
Jiang, Zhi Wen .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2013, 705 (1-3) :26-34
[44]   Antiproliferative Effects of Cucurbitacin B in Breast Cancer Cells: Down-Regulation of the c-Myc/hTERT/Telomerase Pathway and Obstruction of the Cell Cycle [J].
Duangmano, Suwit ;
Dakeng, Sumana ;
Jiratchariyakul, Weena ;
Suksamrarn, Apichart ;
Smith, Duncan R. ;
Patmasiriwat, Pimpicha .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2010, 11 (12) :5323-5338
[45]   Down-regulation of DNA methyltransferase 3B in staurosporine-induced apoptosis and its mechanism in human hepatocarcinoma cell lines [J].
Zhao, Chao ;
Yin, Peng ;
Mei, Chuanzhong ;
Li, Na ;
Yao, Wantong ;
Li, Xin ;
Qi, Jingjing ;
Fan, Kun ;
Li, Zengxia ;
Wang, Liying ;
Shi, Yinghong ;
Qiu, Shuangjian ;
Fan, Jia ;
Zha, Xiliang .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2013, 376 (1-2) :111-119
[46]   MT1-MMP protects breast carcinoma cells against type I collagen-induced apoptosis [J].
E Maquoi ;
D Assent ;
J Detilleux ;
C Pequeux ;
J-M Foidart ;
A Noël .
Oncogene, 2012, 31 :480-493
[47]   Increased expression of dermatopontin mRNA in the infarct zone of experimentally induced myocardial infarction in rats: comparison with decorin and type I collagen mRNAs [J].
Takemoto, S ;
Murakami, T ;
Kusachi, S ;
Iwabu, A ;
Hirohata, S ;
Nakamura, K ;
Sezaki, S ;
Hayashi, J ;
Suezawa, C ;
Ninomiya, Y ;
Tsuji, T .
BASIC RESEARCH IN CARDIOLOGY, 2002, 97 (06) :461-468
[48]   In vitro inhibitory properties of sesquiterpenes from Chloranthus serratus on cell motility via down-regulation of LIMK1 activation in human breast cancer [J].
Fu, Jianjiang ;
Yu, Juanjuan ;
Chen, Jie ;
Xu, Huanjun ;
Luo, Yongming ;
Lu, Hong .
PHYTOMEDICINE, 2018, 49 :23-31
[49]   Human breast cancer cell metastasis is attenuated by lysyl oxidase inhibitors through down-regulation of focal adhesion kinase and the paxillin-signaling pathway [J].
Chen, Li-Ching ;
Tu, Shih-Hsin ;
Huang, Ching-Shui ;
Chen, Ching-Shyang ;
Ho, Chi-Tang ;
Lin, Hsiao-Wei ;
Lee, Chia-Hwa ;
Chang, Hui-Wen ;
Chang, Chien-Hsi ;
Wu, Chih-Hsiung ;
Lee, Wen-Sen ;
Ho, Yuan-Soon .
BREAST CANCER RESEARCH AND TREATMENT, 2012, 134 (03) :989-1004
[50]   Human breast cancer cell metastasis is attenuated by lysyl oxidase inhibitors through down-regulation of focal adhesion kinase and the paxillin-signaling pathway [J].
Li-Ching Chen ;
Shih-Hsin Tu ;
Ching-Shui Huang ;
Ching-Shyang Chen ;
Chi-Tang Ho ;
Hsiao-Wei Lin ;
Chia-Hwa Lee ;
Hui-Wen Chang ;
Chien-Hsi Chang ;
Chih-Hsiung Wu ;
Wen-Sen Lee ;
Yuan-Soon Ho .
Breast Cancer Research and Treatment, 2012, 134 :989-1004